Catalog Number - 82061-25P-4-3M

Cogent NP Silica™ Wide Pore HPLC Columns are designed for the separation of large hydrophilic and polar compounds using both HILIC and Normal Phase chromatography. Manufactured from ultra-high purity, low metal content 300Å silica that remains completely unmodified, this stationary phase provides the native silica selectivity required for proteins, peptides, biomolecules, and other high molecular weight analytes. The wide pore structure improves analyte accessibility to the silica surface, making it highly effective for large molecule separations and method development.

82061-25P-4-3M is packed with 10 µm NP Silica™ Wide Pore particles in a 4.0 mm ID x 250 mm analytical format. The larger particle size generates significantly lower operating backpressure than smaller particle columns, allowing higher flow rates while maintaining reliable chromatographic performance. The 250 mm length provides maximum retention, peak capacity, and chromatographic resolution, making it ideal for challenging large molecule separations and complex sample analyses.

The 10 µm particle size makes this column particularly valuable for preparative method development, purification method scouting, and process scale-up studies. The 300Å wide pore structure provides improved access for proteins, peptides, and other large molecules, while the stationary phase chemistry remains consistent across analytical, preparative, and purification column formats. Methods developed on this column can often be scaled directly to larger internal diameter columns with predictable chromatographic behavior, helping reduce development time and simplify transfer to preparative and purification workflows.

Key Benefits

• NP Silica™ Wide Pore stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 300Å wide pore design

• 10 µm particle size

• Lower operating backpressure

• Ideal for preparative method scouting

• Suitable for HILIC and Normal Phase chromatography

Typical Applications

• Protein analysis

• Peptide analysis

• Biomolecule separations

• Large molecule analysis

• HILIC chromatography

• Normal Phase chromatography

• Preparative method development

• Purification method scouting

• Process scale-up studies

• Method transfer to larger ID columns

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Silica™ Wide Pore

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 10 µm

• Pore Size: 300Å

• Column Dimensions: 4.0 mm ID x 250 mm

• Recommended Flow Rate: 0.8–2.5 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent chemistry

• Unmodified silica stationary phase

• Manufactured from ultra-high purity, low metal content silica

• Designed for proteins, peptides, biomolecules, and other high molecular weight compounds

• Wide pore 300Å silica provides improved accessibility for large analytes

• Lower operating pressure than smaller particle columns

• Supports higher flow rates than 3 µm and 5 µm particle columns

• Compatible with conventional analytical HPLC systems

• Frequently used for preparative chromatography scouting and purification method development

• Excellent for process scale-up studies

• Methods can often be transferred directly to larger preparative and purification columns using the same stationary phase

• 250 mm length provides maximum retention and chromatographic resolution for complex separations

• Ideal for large molecule method development and purification workflows

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.