Semi Prep HPLC Column, NP Diol Wide Pore, 10um, 300A. 10mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Diol™ Wide Pore Semi-Prep HPLC Columns are designed for purification of larger polar and hydrophilic compounds using Normal Phase and HILIC chromatographic modes. Manufactured using ultra-high purity silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention characteristics and selectivity for challenging compounds that require greater pore accessibility than conventional materials. NP Diol™ Wide Pore is often selected as an alternative to unmodified silica columns when purifying larger molecules.
82060-SP50-3M is packed with 10 µm NP Diol™ Wide Pore particles in a 10 mm ID x 50 mm semi-preparative format with a 300Å pore size. The 10 µm particle size is optimized for semi-preparative chromatography, providing lower operating backpressure while allowing higher flow rates and increased sample loading compared to analytical columns. The 300Å pore structure improves analyte accessibility for larger molecules, while the short 50 mm column length is ideal for rapid purification, fraction screening, and method development.
The NP Diol™ Wide Pore stationary phase is non-end-capped and is ideal for purification of larger hydrophilic and polar compounds using either Normal Phase or HILIC conditions. The semi-preparative column format provides an effective balance between purification capacity and solvent consumption, making it suitable for laboratory-scale purification and scale-up studies. These columns are frequently selected for purification of peptides, oligosaccharides, and other larger polar compounds.
Key Benefits
NP Diol™ Wide Pore stationary phase
Ultra-high purity silica support
Terminal diol functional group
Non-end-capped chemistry
300Å wide pore structure
10 µm semi-preparative particle size
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced accessibility for larger molecules
Ideal for rapid purification and method development
Typical Applications
Semi-preparative HPLC
Large molecule purification
HILIC purification
Normal Phase purification
Polar compound isolation
Hydrophilic compound purification
Peptide purification
Oligosaccharide purification
Method development
Fraction collection applications
Specifications
Product Type: Semi-Prep HPLC Column
Stationary Phase: NP Diol™ Wide Pore
Chromatography Mode: Normal Phase / HILIC
Particle Size: 10 µm
Pore Size: 300Å
Column Dimensions: 10 mm ID x 50 mm
Recommended Flow Rate: 3–8 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Designed for purification of larger hydrophilic and polar compounds
Wide pore structure improves accessibility for larger analytes
Alternative to unmodified silica columns
Higher loading capacity than analytical columns
10 µm particles provide lower backpressure at semi-preparative flow rates
10 mm ID format requires a semi-preparative capable HPLC system
Short 50 mm length enables rapid purification and method development
Intended for compound isolation and purification workflows
This is a Special Order Item