Process HPLC Column, NP Diol, Wide Pore, 10um, 300A. 50mm ID x 250mm Length. Cogent Brand. 1 EA.

Additional Information:

Cogent NP Diol™ Wide Pore Process HPLC Columns are designed for high-capacity purification of larger polar and hydrophilic compounds using Normal Phase and HILIC chromatographic modes. Manufactured using ultra-high purity silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention characteristics and selectivity for challenging compounds that require greater pore accessibility than conventional materials. NP Diol™ Wide Pore is often selected as an alternative to unmodified silica columns when purifying larger molecules.

82060-P50-2503M is packed with 10 µm NP Diol™ Wide Pore particles in a 50 mm ID x 250 mm process-scale format with a 300Å pore size. The 10 µm particle size is optimized for high-throughput purification, providing lower operating backpressure while allowing high mobile phase flow rates and substantial sample loading capacity. The combination of a 300Å pore structure, 50 mm internal diameter, and 250 mm length provides maximum loading capacity, increased peak capacity, and enhanced chromatographic resolution for demanding large-molecule purification applications.

The NP Diol™ Wide Pore stationary phase is non-end-capped and is ideal for purification of larger hydrophilic and polar compounds using either Normal Phase or HILIC conditions. The 300Å pore size improves analyte accessibility for larger molecules, while the 50 mm ID format supports efficient scale-up, fraction collection, and production-scale purification workflows. This column is frequently selected when maximum loading capacity, high resolution, and purification of large molecules are required.

Key Benefits

  • NP Diol™ Wide Pore stationary phase

  • Ultra-high purity silica support

  • Terminal diol functional group

  • Non-end-capped chemistry

  • 300Å wide pore structure

  • 10 µm process-scale particle size

  • Suitable for Normal Phase chromatography

  • Suitable for HILIC applications

  • Very high sample loading capacity

  • Optimized for large molecule purification

Typical Applications

  • Process HPLC

  • Production-scale purification

  • Large molecule purification

  • HILIC purification

  • Normal Phase purification

  • Polar compound isolation

  • Hydrophilic compound purification

  • Oligosaccharide purification

  • Process scale-up

  • Fraction collection applications

Specifications

  • Product Type: Process HPLC Column

  • Stationary Phase: NP Diol™ Wide Pore

  • Chromatography Mode: Normal Phase / HILIC

  • Particle Size: 10 µm

  • Pore Size: 300Å

  • Column Dimensions: 50 mm ID x 250 mm

  • Recommended Flow Rate: 80–150 mL/min

  • Column Hardware: Stainless Steel

  • Brand: Cogent

Special Order Item Information

Important Notes

  • Suitable for Normal Phase chromatography

  • Suitable for HILIC applications

  • Designed for purification of larger hydrophilic and polar compounds

  • Wide pore structure improves accessibility for larger analytes

  • Alternative to unmodified silica columns

  • Very high loading capacity for process-scale work

  • 10 µm particles provide lower backpressure at elevated flow rates

  • 50 mm ID format requires a specialized process or large-scale preparative HPLC system

  • 250 mm length provides maximum retention, resolution, and loading capacity

  • Intended for compound isolation and purification workflows

  • This is a Special Order Item


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