Catalog Number - 75025-P50

Cogent HPS Cyano™ Preparative HPLC Columns are designed for high-capacity purification of polar and moderately retained compounds using reversed-phase chromatography. Manufactured using a monofunctional three-carbon ligand with a terminal cyano functional group bonded to high-purity 120Å silica and fully end capped, this stationary phase provides excellent reproducibility, moderate hydrophobicity, and rapid equilibration characteristics. HPS Cyano™ is especially useful when a purification requires less retention than C18 phases while maintaining excellent selectivity and recovery.

75025-P50 is packed with 5 µm HPS Cyano™ particles in a 21.2 mm ID x 50 mm preparative format. The 5 µm particle size provides excellent chromatographic efficiency with lower operating backpressure than smaller particles, while the large 21.2 mm internal diameter delivers substantial sample loading capacity for preparative purification. The short 50 mm length is optimized for rapid purification, high-throughput fraction collection, and preparative method development where productivity and speed are priorities.

The HPS Cyano™ stationary phase is particularly useful for purification of polar and neutral compounds where shorter retention times and faster equilibration are desired. Compared to non-modified silica columns, the cyano phase provides more reproducible reversed-phase behavior while maintaining efficient purification performance. This column is frequently selected for preparative separations requiring rapid turnaround, efficient recovery, and scalable purification methods.

Key Benefits

• HPS Cyano™ stationary phase

• Fully end capped

• Monofunctional cyano bonded phase

• High-purity 120Å silica

• 5 µm preparative particle size

• Moderate retention characteristics

• Faster equilibration between runs

• Lower operating backpressure

• High loading capacity for purification

• Ideal for rapid preparative separations

Typical Applications

• Preparative HPLC

• Polar compound purification

• Neutral compound purification

• Fraction collection

• Pharmaceutical purification

• Method scale-up

• Process development

• Compound isolation

• Sample purification

• Reversed-phase chromatography

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: HPS Cyano™

• Chromatography Mode: Reversed Phase

• Particle Size: 5 µm

• Pore Size: 120Å

• Column Dimensions: 21.2 mm ID x 50 mm

• Recommended Flow Rate: 15–30 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for reversed-phase chromatography

• Fully end capped stationary phase

• Designed for purification of polar and neutral compounds

• Provides faster equilibration than non-modified silica columns

• Offers shorter retention than more hydrophobic phases

• High loading capacity for preparative applications

• 21.2 mm ID format requires a specialized preparative HPLC system

• Recommended flow rate range is approximately 15–30 mL/min

• 50 mm length enables rapid purification and method development

• Frequently compared with cyano columns from Inertsil™ and Luna™ product families

• This is a Special Order Item