Catalog Number - 75008-SP250

Cogent HPS C8™ Semi-Preparative HPLC Columns are designed for purification, fraction collection, and method scale-up of hydrophobic and neutral compounds using reversed-phase chromatography. Manufactured using a unique 120Å silica support with monofunctional octyl (C8) bonding and full double end capping, this stationary phase provides excellent reproducibility, peak shape, and loading capacity. HPS C8™ is widely used as an alternative for USP L7 methods and for developing new purification methods that require moderate hydrophobic retention.

75008-SP250 is packed with 5 µm HPS C8™ particles in a 10 mm ID x 250 mm semi-preparative format. The 5 µm particle size provides excellent chromatographic efficiency while maintaining lower operating backpressure than smaller particles. The 10 mm internal diameter increases sample loading capacity compared to analytical columns, while the 250 mm length provides maximum resolving power and peak capacity for demanding purification applications and method scale-up.

The HPS C8™ stationary phase is ideal for hydrophobic and neutral compounds analyzed using reversed-phase chromatography. Compared to C18 columns, the octyl phase generally provides shorter retention while maintaining excellent selectivity. This column is particularly useful for long-chain fatty acids and other compounds that benefit from moderate hydrophobic interactions. The 250 mm length is often selected when maximum separation performance is required for complex sample mixtures and difficult purifications.

Key Benefits

• HPS C8™ stationary phase

• USP L7 equivalent chemistry

• Monofunctional octyl (C8) bonding

• Fully double end capped

• Unique 120Å silica support

• 5 µm particle size

• Lower operating backpressure

• Shorter retention than C18 phases

• Excellent for long-chain fatty acids

• Maximum resolution for purification and scale-up

Typical Applications

• Semi-preparative HPLC

• Compound purification

• Fraction collection

• Fatty acid purification

• Hydrophobic compound separations

• Neutral compound purification

• Pharmaceutical purification

• Method development

• Method scale-up

• Reversed-phase chromatography

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: HPS C8™ Octyl

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Particle Size: 5 µm

• Pore Size: 120Å

• Column Dimensions: 10 mm ID x 250 mm

• Recommended Flow Rate: 8–15 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for reversed-phase chromatography

• Fully double end capped stationary phase

• Provides shorter retention than C18 columns

• Excellent for long-chain fatty acid purification

• Well suited for hydrophobic and neutral compounds

• Increased loading capacity compared to analytical columns

• 10 mm ID format requires a semi-preparative capable HPLC system

• 250 mm length provides maximum retention, resolution, and peak capacity

• Ideal for purification, isolation, and method scale-up

• Packed using 5 µm particles for efficiency and lower backpressure

• This is a Special Order Item