Catalog Number - 75008-P250

Cogent HPS C8™ Preparative HPLC Columns are designed for high-capacity purification of hydrophobic and neutral compounds using reversed-phase chromatography. Manufactured using a unique 120Å silica support with monofunctional octyl (C8) bonding and full double end capping, this stationary phase provides excellent reproducibility, peak shape, and loading capacity. HPS C8™ is widely used as an alternative for USP L7 methods and for the development of new preparative purification methods requiring moderate hydrophobic retention.

75008-P250 is packed with 5 µm HPS C8™ particles in a 21.2 mm ID x 250 mm preparative format. The 5 µm particle size provides excellent chromatographic efficiency while maintaining lower operating backpressure than smaller particles. The large 21.2 mm internal diameter combined with the extended 250 mm length provides exceptional sample loading capacity, retention, and resolving power for demanding purification applications and preparative method scale-up.

The HPS C8™ stationary phase is ideal for hydrophobic and neutral compounds analyzed using reversed-phase chromatography. Compared to C18 columns, the octyl phase generally produces shorter retention, which can reduce run times while maintaining effective separations. This column is particularly useful for long-chain fatty acids and is frequently selected for preparative purification where maximum resolution, loading capacity, and recovery are required.

Key Benefits

• HPS C8™ stationary phase

• USP L7 equivalent chemistry

• Monofunctional octyl (C8) bonding

• Fully double end capped

• Unique 120Å silica support

• 5 µm preparative particle size

• Lower operating backpressure

• Shorter retention than C18 phases

• Excellent for long-chain fatty acids

• Maximum loading capacity and preparative resolution

Typical Applications

• Preparative HPLC

• Compound purification

• Fraction collection

• Fatty acid purification

• Hydrophobic compound separations

• Neutral compound purification

• Pharmaceutical purification

• Method scale-up

• Process development

• Reversed-phase chromatography

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: HPS C8™ Octyl

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Particle Size: 5 µm

• Pore Size: 120Å

• Column Dimensions: 21.2 mm ID x 250 mm

• Recommended Flow Rate: 15–30 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for reversed-phase chromatography

• Fully double end capped stationary phase

• Provides shorter retention than C18 columns

• Excellent for long-chain fatty acid purification

• Well suited for hydrophobic and neutral compounds

• High loading capacity for preparative applications

• 21.2 mm ID format requires a specialized preparative HPLC system

• 250 mm length provides maximum retention, resolution, and peak capacity

• Ideal for complex purifications and preparative method scale-up

• Packed using 5 µm particles for efficiency and lower backpressure

• This is a Special Order Item