Catalog Number - 68540-SP50-3M

Cogent NP Amino™ Wide Pore Semi-Preparative HPLC Columns are designed for purification, method development, and scale-up of larger polar compounds using Normal Phase and HILIC chromatographic modes. Manufactured using the highest purity silica available bonded with a three-carbon propyl ligand containing a terminal amino functional group, this stationary phase provides unique selectivity for negatively charged and highly polar analytes. NP Amino™ Wide Pore is widely used as an alternative for USP L8 methods when analyzing or purifying larger molecules that benefit from enhanced pore accessibility.

68540-SP50-3M is packed with 5 µm NP Amino™ Wide Pore particles in a 10 mm ID x 50 mm semi-preparative format with a 300Å pore size. The 5 µm particle size provides excellent chromatographic efficiency while supporting higher sample loading than analytical columns. The 300Å wide pore structure improves accessibility for larger molecules, while the 10 mm internal diameter provides increased loading capacity for purification and method scale-up applications. The short 50 mm column length is ideal for rapid separations, high-throughput workflows, and fast method development.

The NP Amino™ Wide Pore stationary phase is not end capped and is optimized for complex sugars, compounds containing carboxylic acid functionalities, and other larger polar analytes requiring Normal Phase or HILIC retention mechanisms. These columns are packed and shipped in Normal Phase solvents and are frequently selected for transferring analytical methods to semi-preparative purification workflows involving larger molecules where speed and productivity are priorities.

Key Benefits

• NP Amino™ Wide Pore stationary phase

• USP L8 equivalent chemistry

• Propyl amino bonded silica

• Not end capped

• 300Å wide pore structure

• 5 µm particle size

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Enhanced accessibility for larger molecules

• Ideal for rapid purification and method scale-up

Typical Applications

• Semi-preparative HPLC

• Complex sugar purification

• Carbohydrate isolation

• Organic acid separations

• HILIC chromatography

• Normal Phase chromatography

• Large molecule purification

• Method development

• Method scale-up

• Fraction collection

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: NP Amino™ Wide Pore

• Chromatography Mode: Normal Phase / HILIC

• USP Classification: L8

• Particle Size: 5 µm

• Pore Size: 300Å

• Column Dimensions: 10 mm ID x 50 mm

• Recommended Flow Rate: 4–8 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• USP L8 equivalent chemistry

• Designed for complex sugars and larger polar compounds

• Ideal for compounds containing carboxylic acid groups

• Propyl amino bonded stationary phase

• Wide pore structure improves accessibility for larger analytes

• Increased loading capacity for purification workflows

• 10 mm ID format requires a semi-preparative capable HPLC system

• 50 mm length enables rapid separations and method development

• Shipped in Normal Phase solvents

• This is a Special Order Item

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