Catalog Number - 68540-SP250

Cogent NP Amino™ Semi-Preparative HPLC Columns are designed for purification, method development, and scale-up of polar compounds using Normal Phase and HILIC chromatographic modes. Manufactured using world-class silica bonded with a three-carbon propyl ligand containing a terminal amino functional group, this stationary phase provides unique selectivity for negatively charged and highly polar analytes. NP Amino™ is widely used as an alternative for USP L8 methods and for new method development involving carbohydrates, organic acids, and other polar compounds.

68540-SP250 is packed with 5 µm NP Amino™ particles in a 10 mm ID x 250 mm semi-preparative format with a 100Å pore size. The 5 µm particle size delivers excellent chromatographic efficiency while supporting higher sample loading than analytical columns. The 10 mm internal diameter provides increased loading capacity for purification and method scale-up applications, while the 250 mm column length delivers maximum resolving power and peak capacity for the most demanding separations.

The NP Amino™ stationary phase is not end capped and is commonly used for compounds containing carboxylic acid functionalities, sugars, and other polar analytes that require Normal Phase or HILIC retention mechanisms. These columns are packed and shipped in Normal Phase solvents and are frequently selected for transferring analytical methods to semi-preparative purification workflows requiring maximum resolution.

Key Benefits

• NP Amino™ stationary phase

• USP L8 equivalent chemistry

• Propyl amino bonded silica

• Not end capped

• 100Å pore size

• 5 µm particle size

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Increased loading capacity versus analytical columns

• Maximum resolution for purification and scale-up

Typical Applications

• Semi-preparative HPLC

• Sugar purification

• Carbohydrate isolation

• Organic acid separations

• HILIC chromatography

• Normal Phase chromatography

• Polar compound purification

• Method development

• Method scale-up

• Fraction collection

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: NP Amino™

• Chromatography Mode: Normal Phase / HILIC

• USP Classification: L8

• Particle Size: 5 µm

• Pore Size: 100Å

• Column Dimensions: 10 mm ID x 250 mm

• Recommended Flow Rate: 8–15 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• USP L8 equivalent chemistry

• Designed for sugars and other polar compounds

• Ideal for compounds containing carboxylic acid groups

• Propyl amino bonded stationary phase

• Increased loading capacity for purification workflows

• 10 mm ID format requires a semi-preparative capable HPLC system

• 250 mm length provides maximum retention, resolution, and peak capacity

• Shipped in Normal Phase solvents

• This is a Special Order Item

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