Catalog Number - 68540-P50-250

Cogent NP Amino™ Process HPLC Columns are designed for high-capacity purification of polar compounds using Normal Phase and HILIC chromatographic modes. Manufactured using world-class silica bonded with a three-carbon propyl ligand containing a terminal amino functional group, this stationary phase provides unique selectivity for negatively charged and highly polar analytes. NP Amino™ is widely used for process-scale separations involving carbohydrates, organic acids, and other polar compounds that can be difficult to retain using conventional reversed-phase chromatography.

68540-P50-250 is packed with 5 µm NP Amino™ particles in a 50 mm ID x 250 mm process-scale format with a 100Å pore size. The 5 µm particle size provides excellent chromatographic efficiency and resolution while maintaining the very high loading capacity required for process-scale purification. The extended 250 mm column length delivers maximum resolving power and peak capacity, making it ideal for demanding purification workflows, process development, and manufacturing-scale applications.

The NP Amino™ stationary phase is not end capped and is commonly used for purification of sugars, compounds containing carboxylic acid functionalities, and other negatively charged or highly polar analytes. These columns are suitable for both Normal Phase and HILIC separations and are shipped in Normal Phase solvents. The large-diameter process format enables efficient scale-up from preparative methods while supporting high-throughput purification and production operations.

Key Benefits

• NP Amino™ stationary phase

• Propyl amino bonded silica

• Not end capped

• 100Å pore size

• 5 µm process-scale particle size

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Very high sample loading capacity

• Excellent for polar and negatively charged compounds

• Maximum resolution for process-scale purification

Typical Applications

• Process HPLC

• Sugar purification

• Carbohydrate isolation

• Organic acid purification

• HILIC purification

• Normal Phase purification

• Polar compound isolation

• Process development

• Pilot-scale purification

• Manufacturing support purification

Specifications

• Product Type: Process HPLC Column

• Stationary Phase: NP Amino™

• Chromatography Mode: Normal Phase / HILIC

• Particle Size: 5 µm

• Pore Size: 100Å

• Column Dimensions: 50 mm ID x 250 mm

• Recommended Flow Rate: 80–150 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Designed for purification of sugars and other polar compounds

• Ideal for compounds containing carboxylic acid groups

• Propyl amino bonded stationary phase

• Very high loading capacity for process-scale work

• 5 µm particles provide enhanced chromatographic efficiency and resolution

• 50 mm ID format requires a specialized process HPLC system

• 250 mm length provides maximum retention, resolution, and peak capacity

• Shipped in Normal Phase solvents

• Intended for large-scale compound isolation and purification workflows

• This is a Special Order Item

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