Catalog Number - 68540-P21-2503M

Cogent NP Amino™ Wide Pore Preparative HPLC Columns are designed for high-capacity purification of larger polar compounds using Normal Phase and HILIC chromatographic modes. Manufactured using the highest purity silica available bonded with a three-carbon propyl ligand containing a terminal amino functional group, this stationary phase provides unique selectivity for negatively charged and highly polar analytes. NP Amino™ Wide Pore is particularly useful for purification of larger sugars, complex carbohydrates, and compounds containing carboxylic acid functionalities.

68540-P21-2503M is packed with 5 µm NP Amino™ Wide Pore particles in a 21.2 mm ID x 250 mm preparative format with a 300Å pore size. The 5 µm particle size provides excellent chromatographic efficiency and resolution, while the 300Å pore structure improves analyte accessibility for larger molecules. The extended 250 mm column length provides maximum resolving power, increased peak capacity, and superior separation performance for challenging preparative purifications.

The NP Amino™ Wide Pore stationary phase is not end capped and is optimized for purification of larger polar compounds using either Normal Phase or HILIC conditions. The wide pore structure enhances performance for complex sugars and larger molecules containing carboxylic acid groups. These columns are packed and shipped in Normal Phase solvents and are frequently selected for preparative purification when maximum retention, resolution, and pore accessibility are required.

Key Benefits

• NP Amino™ Wide Pore stationary phase

• Propyl amino bonded silica

• Not end capped

• 300Å wide pore structure

• 5 µm preparative particle size

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• High sample loading capacity

• Enhanced accessibility for larger molecules

• Maximum resolution for preparative purification

Typical Applications

• Preparative HPLC

• Complex sugar purification

• Carbohydrate isolation

• Organic acid purification

• HILIC purification

• Normal Phase purification

• Large molecule purification

• Fraction collection

• Method scale-up

• Compound purification

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: NP Amino™ Wide Pore

• Chromatography Mode: Normal Phase / HILIC

• Particle Size: 5 µm

• Pore Size: 300Å

• Column Dimensions: 21.2 mm ID x 250 mm

• Recommended Flow Rate: 15–30 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Designed for larger sugars and polar compounds

• Ideal for compounds containing carboxylic acid groups

• Propyl amino bonded stationary phase

• Wide pore structure improves accessibility for larger analytes

• High loading capacity for preparative work

• 5 µm particles provide enhanced chromatographic efficiency and resolution

• 21.2 mm ID format requires a specialized preparative HPLC system

• 250 mm length provides maximum retention, resolution, and peak capacity

• Shipped in Normal Phase solvents

• Intended for compound isolation and purification workflows

• This is a Special Order Item