Catalog Number - 68540-P21-250

Cogent NP Amino™ Preparative HPLC Columns are designed for high-capacity purification of polar compounds using Normal Phase and HILIC chromatographic modes. Manufactured using world-class silica bonded with a three-carbon propyl ligand containing a terminal amino functional group, this stationary phase provides unique selectivity for negatively charged and highly polar analytes. NP Amino™ is widely used for preparative separations involving carbohydrates, organic acids, and other polar compounds that can be difficult to retain using conventional reversed-phase chromatography.

68540-P21-250 is packed with 5 µm NP Amino™ particles in a 21.2 mm ID x 250 mm preparative format with a 100Å pore size. The 5 µm particle size provides improved chromatographic efficiency and resolution while maintaining the sample loading capacity required for preparative purification. The extended 250 mm column length provides maximum resolving power and increased peak capacity, making it ideal for demanding preparative separations and complex sample purifications.

The NP Amino™ stationary phase is not end capped and is commonly used for purification of sugars, compounds containing carboxylic acid functionalities, and other negatively charged or highly polar analytes. These columns are suitable for both Normal Phase and HILIC separations and are shipped in Normal Phase solvents. The preparative format enables efficient purification and recovery of target compounds while supporting scale-up from analytical methods.

Key Benefits

• NP Amino™ stationary phase

• Propyl amino bonded silica

• Not end capped

• 100Å pore size

• 5 µm preparative particle size

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• High sample loading capacity

• Excellent for polar and negatively charged compounds

• Maximum resolution for preparative purification

Typical Applications

• Preparative HPLC

• Sugar purification

• Carbohydrate isolation

• Organic acid purification

• HILIC purification

• Normal Phase purification

• Polar compound isolation

• Fraction collection

• Method scale-up

• Compound purification

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: NP Amino™

• Chromatography Mode: Normal Phase / HILIC

• Particle Size: 5 µm

• Pore Size: 100Å

• Column Dimensions: 21.2 mm ID x 250 mm

• Recommended Flow Rate: 15–30 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Designed for purification of sugars and other polar compounds

• Ideal for compounds containing carboxylic acid groups

• Propyl amino bonded stationary phase

• High loading capacity for preparative work

• 5 µm particles provide enhanced chromatographic efficiency and resolution

• 21.2 mm ID format requires a specialized preparative HPLC system

• 250 mm length provides maximum retention, resolution, and peak capacity

• Shipped in Normal Phase solvents

• Intended for compound isolation and purification workflows

• This is a Special Order Item