HPLC Column, NP Amino, 5um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA. Special Order Item.

Additional Information:

Cogent NP Amino™ HPLC Columns are designed for Normal Phase and HILIC separations of highly polar compounds. Manufactured using ultra-high purity silica bonded with a three-carbon propyl linker and terminal amino functionality, this stationary phase provides the selectivity needed for retention and separation of compounds that can be difficult to retain using traditional reversed-phase chromatography. NP Amino™ is an excellent alternative for USP L8 methods and for new method development involving polar analytes.

68540-25P-4 is packed with 5 µm NP Amino™ particles in a 4.0 mm ID x 250 mm format with a 100Å pore size. The 5 µm particle size provides reliable performance with moderate backpressure while maintaining excellent reproducibility. The extended 250 mm column length provides maximum resolving power and increased peak capacity, making it ideal for complex separations, method optimization, and analytical methods requiring enhanced retention and resolution.

The amino stationary phase is not end capped and is particularly useful for sugars, carbohydrates, organic acids, and other highly polar compounds containing hydroxyl or carboxylic acid functionalities. This column can be used in Normal Phase and HILIC chromatographic modes and is packed and shipped in normal-phase solvents. The extended column length provides excellent retention and separation of challenging analytes while offering an effective alternative retention mechanism to reversed-phase chromatography.

Key Benefits

  • NP Amino™ stationary phase

  • USP L8 equivalent chemistry

  • Ultra-high purity silica support

  • Three-carbon propyl linker with terminal amino group

  • Not end capped

  • 100Å pore size

  • Suitable for Normal Phase chromatography

  • Suitable for HILIC applications

  • Excellent retention of highly polar compounds

  • Maximum resolution for demanding separations

Typical Applications

  • Normal Phase HPLC

  • HILIC chromatography

  • USP L8 methods

  • Sugar analysis

  • Carbohydrate separations

  • Organic acid analysis

  • Polar compound separations

  • Food and beverage testing

  • Pharmaceutical analysis

  • Method development

Specifications

  • Product Type: HPLC Column

  • Stationary Phase: NP Amino™

  • Chromatography Mode: Normal Phase / HILIC

  • USP Classification: L8

  • Particle Size: 5 µm

  • Pore Size: 100Å

  • Column Dimensions: 4.0 mm ID x 250 mm

  • Recommended Flow Rate: 0.8–1.5 mL/min

  • Column Hardware: Stainless Steel

  • Brand: Cogent

Special Order Item Information

Important Notes

  • Suitable for Normal Phase chromatography

  • Suitable for HILIC applications

  • USP L8 equivalent chemistry

  • Not end capped

  • Designed for separation of highly polar compounds

  • Frequently used for sugars and compounds containing carboxylic acid groups

  • Packed and shipped in normal-phase solvents

  • 250 mm length provides maximum retention and resolution

  • Provides an alternative retention mechanism to reversed-phase chromatography

  • This is a Special Order Item


 

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