Catalog Number - 68534-25P-4-3M

Cogent RP Cyano™ Wide Pore HPLC Columns are designed for reversed-phase separations of larger polar and neutral compounds where alternative selectivity to traditional alkyl-bonded phases is desired. Manufactured using ultra-high purity silica bonded with a three-carbon ligand and terminal Cyano functionality, this stationary phase provides unique chromatographic characteristics that make it an excellent choice for USP L10 methods involving larger molecules. The 300Å wide pore structure improves analyte accessibility while maintaining the distinctive selectivity of RP Cyano™ chemistry.

68534-25P-4-3M is packed with 5 µm RP Cyano™ Wide Pore particles in a 4.0 mm ID x 250 mm format with a 300Å pore size. The 5 µm particle size provides lower operating backpressure than smaller particles while maintaining excellent efficiency and reproducibility. The extended 250 mm column length provides maximum resolving power and increased peak capacity, making it ideal for complex separations involving proteins, peptides, enzymes, natural products, and other large molecules.

The RP Cyano™ Wide Pore stationary phase is not end capped and is frequently used for proteins, peptides, enzymes, natural products, and other larger polar or neutral compounds in reversed-phase HPLC. Compared to non-modified silica columns, it provides shorter retention times and faster equilibration between runs. Packed and shipped in reversed-phase solvents. See Cogent NP Cyano Columns Packed in Normal-Phase Solvents for applications requiring normal-phase chromatography.

Key Benefits

• RP Cyano™ Wide Pore stationary phase

• USP L10 equivalent chemistry

• Ultra-high purity silica support

• Three-carbon linker with terminal Cyano group

• Not end capped

• 300Å pore size

• Suitable for reversed-phase chromatography

• Enhanced accessibility for larger molecules

• Faster equilibration between runs

• Maximum resolution for complex separations

Typical Applications

• Reversed-phase HPLC

• USP L10 methods

• Protein analysis

• Peptide separations

• Enzyme analysis

• Natural product analysis

• Polar compound separations

• Neutral compound separations

• Method development

• Complex sample separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Cyano™ Wide Pore

• Chromatography Mode: Reversed Phase

• USP Classification: L10

• Particle Size: 5 µm

• Pore Size: 300Å

• Column Dimensions: 4.0 mm ID x 250 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for reversed-phase chromatography

• USP L10 equivalent chemistry

• Not end capped

• Designed for proteins, peptides, enzymes, and other larger molecules

• Wide pore structure improves accessibility for large analytes

• Provides alternative selectivity compared to C18 and C8 columns

• Faster equilibration than non-modified silica columns

• 250 mm length provides maximum resolution and peak capacity

• Packed and shipped in reversed-phase solvents

• See Cogent NP Cyano Columns Packed in Normal-Phase Solvents for normal-phase chromatography applications

• This is a Special Order Item