Catalog Number - 68524-SP100

Cogent RP Phenyl™ Semi Prep HPLC Columns are designed for purification, method scale-up, and semi-preparative chromatography involving aromatic compounds and structurally related analytes. Manufactured using ultra-high purity silica bonded with a three-carbon linker and terminal Phenyl functionality, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. RP Phenyl™ offers enhanced aromatic selectivity and is an excellent choice for separations involving compounds containing phenyl rings and related structures.

68524-SP100 is packed with 5 µm RP Phenyl™ particles featuring a 100Å pore size in a 10 mm ID x 100 mm semi-preparative format. The 5 µm particle size performs well at higher flow rates while maintaining moderate operating backpressure. The larger internal diameter provides increased loading capacity compared to analytical columns, making this column ideal for purification, fraction collection, and analytical-to-semi-preparative method scale-up.

The 10 mm ID x 100 mm format is particularly well suited for purification of positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds containing aromatic structures. Typical operating flow rates are approximately 4–8 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A semi-preparative HPLC system capable of supporting these flow rates and sample loads is required.

Key Benefits

• RP Phenyl™ stationary phase

• USP L11 equivalent chemistry

• Ultra-high purity silica support

• Fully double end-capped

• Phenyl bonded phase

• 100Å pore size

• Suitable for reversed-phase chromatography

• Enhanced aromatic selectivity

• Increased sample loading capacity

• Ideal for purification and method scale-up

Typical Applications

• Semi-preparative HPLC

• Purification of positional isomers

• Tocopherol purification

• Flavonoid purification

• Polynuclear aromatic hydrocarbon separations

• Nitro-aromatic compound purification

• Active pharmaceutical ingredient (API) purification

• Natural product isolation

• Fraction collection

• Analytical-to-semi-preparative method scale-up

Specifications

• Product Type: Semi Prep HPLC Column

• Stationary Phase: RP Phenyl™

• Chromatography Mode: Reversed Phase

• USP Classification: L11

• Particle Size: 5 µm

• Pore Size: 100Å

• Column Dimensions: 10 mm ID x 100 mm

• Recommended Flow Rate: 4–8 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for reversed-phase chromatography

• USP L11 equivalent chemistry

• Fully double end-capped stationary phase

• Excellent selectivity for aromatic compounds

• Ideal for purification of compounds containing phenyl ring structures

• Increased loading capacity compared to analytical columns

• Typical operating range is approximately 4–8 mL/min

• Requires a semi-preparative HPLC system capable of reliable operation at semi-prep flow rates

• Well suited for purification, fraction collection, and method scale-up

• This is a Special Order Item