HPLC Column, RP Phenyl, 5um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA. Special Order Item.

Additional Information:

Cogent RP Phenyl™ HPLC Columns are designed for reversed-phase separations where aromatic selectivity is beneficial. Manufactured using ultra-high purity silica bonded with a three-carbon linker and terminal phenyl functionality, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. RP Phenyl™ is an excellent alternative for USP L11 methods and is commonly selected for both established methods and new method development.

68524-25P-4 is packed with 5 µm RP Phenyl™ particles featuring a 100 Å pore size in a 4.0 mm ID x 250 mm format. The 5 µm particle size provides excellent chromatographic efficiency while maintaining moderate operating backpressure. The extended 250 mm column length delivers maximum resolution and peak capacity, making it ideal for complex separations requiring enhanced analyte discrimination and retention.

The 4.0 mm ID x 250 mm format is well suited for aromatic and structurally related compounds in reversed-phase HPLC. Typical flow rates are approximately 0.8-1.2 mL/min depending on mobile phase composition and application requirements. This column format is frequently used for USP monograph methods and applications where maximum chromatographic resolution is required.

Key Benefits

  • RP Phenyl™ stationary phase

  • USP L11 equivalent chemistry

  • Ultra-high purity silica support

  • Fully double end-capped

  • Phenyl bonded phase

  • 100 Å pore size

  • Suitable for reversed-phase HPLC

  • Enhanced aromatic selectivity

  • Excellent reproducibility

  • Maximum resolution and peak capacity

Typical Applications

  • USP monograph methods

  • Positional isomer separations

  • Tocopherol analysis

  • Flavonoid analysis

  • Polynuclear aromatic hydrocarbon analysis

  • Nitro-aromatic compound analysis

  • Active pharmaceutical ingredient (API) analysis

  • Natural product analysis

  • Pharmaceutical quality control

  • Reversed-phase HPLC

Specifications

  • Product Type: HPLC Column

  • Stationary Phase: RP Phenyl™

  • Chromatography Mode: Reversed Phase

  • USP Classification: L11

  • Particle Size: 5 µm

  • Pore Size: 100 Å

  • Column Dimensions: 4.0 mm ID x 250 mm

  • Recommended Flow Rate: 0.8-1.2 mL/min

  • Column Hardware: Stainless Steel

  • Brand: Cogent

Special Order Item Information

Important Notes

  • Suitable for reversed-phase chromatography

  • USP L11 equivalent chemistry

  • Fully double end-capped stationary phase

  • Excellent selectivity for aromatic compounds

  • Useful for separation of positional isomers and structurally similar analytes

  • Commonly used for tocopherols, flavonoids, APIs, and natural products

  • 250 mm length provides maximum resolution and peak capacity

  • Frequently used for USP monograph and demanding pharmaceutical methods

  • Compatible with most standard HPLC systems

  • This is a Special Order Item


 

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