HPLC Column, RP Phenyl, Wide Pore, 3um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP Phenyl™ Wide Pore is a reversed-phase HPLC stationary phase designed to provide unique selectivity through π-π interactions between aromatic analytes and the bonded phenyl functionality while offering enhanced accessibility for larger molecules. Manufactured using a traditional three-carbon linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore phase provides chromatographic behavior distinct from conventional alkyl phases. As a USP L11 equivalent phase, it is particularly useful for large molecules, aromatic compounds, natural products, peptides, and method development applications.
68324-25P-4-3M is an HPLC column packed with 3 µm RP Phenyl™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity within the RP Phenyl product family, while the 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns. The wide pore structure improves analyte access to the bonded phase for larger compounds while maintaining the distinctive selectivity associated with phenyl chemistry. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for the separation of large positional isomers, natural products, related compounds, and certain peptides in reversed-phase HPLC. The unique selectivity of the phenyl phase can provide improved resolution for aromatic analytes where conventional C8 or C18 phases may not deliver adequate selectivity. The combination of wide pores, enhanced aromatic selectivity, and maximum resolving power makes this column an excellent choice for demanding pharmaceutical, bioanalytical, and research applications.
Key Benefits
RP Phenyl™ Wide Pore stationary phase
USP L11 equivalent chemistry
Fully double end capped
Phenyl bonded phase
Ultra-high purity silica support
300 Å wide pore design
Enhanced aromatic selectivity
Optimized for larger molecules
3 µm particle size
Maximum chromatographic resolution
Typical Applications
USP L11 methods
Large positional isomer separations
Natural product analysis
Peptide analysis
Aromatic compound analysis
Method development
Bioanalytical methods
Pharmaceutical analysis
Reversed-phase HPLC
Research and development
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl™ Wide Pore
USP Classification: L11
Chromatography Mode: Reversed Phase
Bonded Phase: Phenyl
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
For complete details, please review the Special Order Item Information.
Important Notes
Designed for larger molecules and aromatic compounds
300 Å wide pores improve access to the bonded phase for larger analytes
Suitable for conventional HPLC systems
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Generates higher backpressure than larger particle columns
The 250 mm length provides maximum resolution and peak capacity for demanding separations
Particularly useful for peptides, natural products, and larger aromatic analytes
Provides unique selectivity compared to C8 and C18 phases
Commonly used as an alternative for USP L11 methods involving larger analytes
Well suited for impurity profiling, method development, and advanced chromatographic optimization
This is a Special Order Item