HPLC Column, RP Phenyl, 3um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP Phenyl™ is a reversed-phase HPLC stationary phase designed to provide unique selectivity through π-π interactions between aromatic analytes and the bonded phenyl functionality. Manufactured using a traditional three-carbon linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this stationary phase provides chromatographic behavior distinct from conventional alkyl phases. As a USP L11 equivalent phase, it is widely used for method replacement, method development, and applications involving aromatic and structurally related compounds.
68324-25P-4 is an HPLC column packed with 3 µm RP Phenyl™ particles featuring a 100 Å pore size in a 4.0 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity within the RP Phenyl product family, while the 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for the separation of positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. The unique selectivity of the phenyl phase often improves aromatic and structural selectivity compared to conventional alkyl phases. The extended 250 mm length provides exceptional resolving power for USP monograph methods, impurity profiling, and highly challenging separations.
Key Benefits
RP Phenyl™ stationary phase
USP L11 equivalent chemistry
Fully double end capped
Phenyl bonded phase
Ultra-high purity silica support
Enhanced aromatic selectivity
Excellent isomer separation capability
3 µm particle size
Greater efficiency than 5 µm columns
Maximum chromatographic resolution
Typical Applications
USP L11 methods
USP monograph methods
Positional isomer separations
Tocopherol analysis
Flavonoid analysis
Polynuclear aromatic hydrocarbon analysis
Nitro-aromatic compound analysis
Active pharmaceutical ingredient (API) analysis
Natural product analysis
Impurity profiling
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl™
USP Classification: L11
Chromatography Mode: Reversed Phase
Bonded Phase: Phenyl
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 100 Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
For complete details, please review the Special Order Item Information.
Important Notes
Suitable for conventional HPLC systems
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Generates higher backpressure than larger particle columns
The 250 mm length provides maximum resolution and peak capacity for demanding separations
Provides unique selectivity compared to C8 and C18 phases
Particularly useful for aromatic compounds and positional isomers
Frequently used in USP monograph methods
Commonly used as an alternative for USP L11 methods
Well suited for impurity profiling, method development, and chromatographic optimization
This is a Special Order Item