HPLC Column, RP Phenyl, Wide Pore, 3um, 4.0mm ID x 100mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.

Additional Information:

Cogent RP Phenyl™ Wide Pore is a reversed-phase HPLC stationary phase designed to provide unique selectivity through π-π interactions between aromatic analytes and the bonded phenyl functionality while offering enhanced accessibility for larger molecules. Manufactured using a traditional three-carbon linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore phase provides chromatographic behavior distinct from conventional alkyl phases. As a USP L11 equivalent phase, it is particularly useful for large molecules, aromatic compounds, natural products, peptides, and method development applications.

68324-10P-4-3M is an HPLC column packed with 3 µm RP Phenyl™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 100 mm format. The 100 mm length provides greater chromatographic resolution than shorter 50 mm columns while maintaining practical analysis times for routine and research applications. The 3 µm particles provide improved efficiency and sharper peaks than comparable 5 µm columns, while the wide pore structure improves analyte access to the bonded phase for larger compounds. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.

This column is particularly useful for the separation of large positional isomers, natural products, related compounds, and certain peptides in reversed-phase HPLC. The unique selectivity of the phenyl phase can provide improved resolution for aromatic analytes where conventional C8 or C18 phases may not deliver adequate selectivity. The combination of wide pores and enhanced aromatic selectivity makes this column an excellent choice for demanding analytical, pharmaceutical, and bioanalytical methods.

Key Benefits

  • RP Phenyl™ Wide Pore stationary phase

  • USP L11 equivalent chemistry

  • Fully double end capped

  • Phenyl bonded phase

  • Ultra-high purity silica support

  • 300 Å wide pore design

  • Enhanced aromatic selectivity

  • Optimized for larger molecules

  • 3 µm particle size

  • Improved chromatographic resolution

Typical Applications

  • USP L11 methods

  • Large positional isomer separations

  • Natural product analysis

  • Peptide analysis

  • Aromatic compound analysis

  • Method development

  • Bioanalytical methods

  • Pharmaceutical analysis

  • Reversed-phase HPLC

  • Research and development

Specifications

  • Product Type: HPLC Column

  • Stationary Phase: RP Phenyl™ Wide Pore

  • USP Classification: L11

  • Chromatography Mode: Reversed Phase

  • Bonded Phase: Phenyl

  • Endcapping: Fully Double End Capped

  • Particle Size: 3 µm

  • Pore Size: 300 Å

  • Column Dimensions: 4.0 mm ID x 100 mm

  • Length: 100 mm

  • Typical Flow Rate: 0.8–1.5 mL/min

  • Brand: Cogent

Special Order Item Information

Important Notes

  • Designed for larger molecules and aromatic compounds

  • 300 Å wide pores improve access to the bonded phase for larger analytes

  • Suitable for conventional HPLC systems

  • 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

  • Generates higher backpressure than larger particle columns

  • The 100 mm length provides greater resolution than 50 mm columns while maintaining practical run times

  • Particularly useful for natural products and peptide separations

  • Provides unique selectivity compared to C8 and C18 phases

  • Commonly used as an alternative for USP L11 methods involving larger analytes

  • This is a Special Order Item


 

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