HPLC Column, RP Phenyl, 10um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Phenyl is MICROSOLV's most popular phenyl stationary phase for reversed-phase HPLC and LC-MS applications. Manufactured with high-purity silica and a C3-phenyl bonded phase featuring exhaustive double endcapping, this column provides balanced hydrophobic retention combined with strong π-π interactions. The result is enhanced selectivity for aromatic, heteroaromatic, and moderately polar compounds that may not separate adequately on traditional C18 or C8 columns. The stationary phase is widely used in QC, GMP, research, and method development laboratories and is well suited as an alternative for USP L11 methods.
68024-25P-4 is an analytical HPLC column with dimensions of 4.0 mm ID x 250 mm length, packed with 10 µm RP Phenyl particles featuring a 100 Å pore size. The 250 mm column length provides maximum resolving power and peak capacity for complex and highly challenging separations. The C3-phenyl bonded phase provides improved selectivity for aromatic compounds through π-π interaction mechanisms while maintaining reproducible retention, excellent peak symmetry, reduced peak tailing, and lower silanol activity. The 10 µm particle size supports higher flow rates while generating lower backpressure than smaller particle columns. Typical flow rates are approximately 0.8–1.8 mL/min depending on mobile phase composition and system configuration.
This column is commonly used for aromatic pharmaceuticals, positional isomers, peptides, tocopherols, flavonoids, polynuclear aromatic compounds, nitro-aromatic compounds, APIs, natural products, and related compounds. It is particularly useful when structurally similar analytes co-elute on C18 columns or when additional aromatic selectivity is desired. The 250 mm length maximizes chromatographic efficiency and is often selected for demanding method development, impurity profiling, and complex sample analyses where the highest possible resolution is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP Phenyl stationary phase with C3-phenyl bonded chemistry
Strong π-π interaction selectivity
Fully double endcapped
High-purity silica support
Reduced silanol activity
Reduced peak tailing
Excellent peak symmetry
Suitable alternative for USP L11 methods
Compatible with HPLC and LC-MS
Excellent for aromatic and moderately polar compounds
Typical Applications
USP L11 methods
Positional isomer separations
Aromatic pharmaceutical compounds
Active pharmaceutical ingredient (API) analysis
Peptide separations
Tocopherol analysis
Flavonoid analysis
Polynuclear aromatic compound analysis
Nitro-aromatic compound analysis
Natural product analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl
Chromatography Mode: Reversed Phase
USP Classification: L11
Bonded Phase: C3-Phenyl (Propyl Phenyl)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 100 Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.8 mL/min
Brand: Cogent
Additional Resources
Special Order Item Information
Important Notes
Suitable as an alternative for USP L11 methods
Often selected when C18 or C8 columns do not provide sufficient selectivity
Excellent for aromatic and structurally similar compounds
Effective for peptides and moderately polar analytes
Commonly used in GMP, QC, and research laboratories
Maximum column length provides the highest resolving power for complex separations
Frequently used for impurity profiling and demanding method development
A Cogent Column Filter is included and recommended to help protect the column and extend service life
Special Order Item