HPLC Column, RP Phenyl, Wide Pore, 10um, 4.0mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Phenyl Wide Pore is MICROSOLV's most popular phenyl stationary phase for reversed-phase HPLC and LC-MS applications involving larger molecules. Manufactured with high-purity silica and a C3-phenyl bonded phase featuring exhaustive double endcapping, this column provides balanced hydrophobic retention combined with strong π-π interactions. The result is enhanced selectivity for aromatic, heteroaromatic, and moderately polar compounds that may not separate adequately on traditional C18 or C8 columns. The 300 Å wide pore structure improves accessibility for larger molecules while maintaining the aromatic selectivity that makes RP Phenyl phases a preferred choice for many challenging separations. This stationary phase is also suitable as an alternative for USP L11 methods.
68024-05P-4-3M is an analytical HPLC column with dimensions of 4.0 mm ID x 50 mm length, packed with 10 µm RP Phenyl Wide Pore particles featuring a 300 Å pore size. The short column length is ideal for rapid analyses, high-throughput screening, and method development workflows. The C3-phenyl bonded phase provides improved selectivity for aromatic compounds through π-π interaction mechanisms while maintaining reproducible retention, excellent peak symmetry, reduced peak tailing, and lower silanol activity. The 10 µm particle size supports higher flow rates while generating lower backpressure than smaller particle columns. Typical flow rates are approximately 0.8-2.0 mL/min depending on mobile phase composition and system configuration.
This column is commonly used for aromatic pharmaceuticals, positional isomers, peptides, tocopherols, flavonoids, polynuclear aromatic compounds, nitro-aromatic compounds, APIs, natural products, and related compounds. The wide pore architecture can offer advantages for larger molecules while preserving the unique phenyl selectivity. It is particularly useful when structurally similar analytes co-elute on C18 columns or when additional aromatic selectivity is desired. The 50 mm length is also frequently selected for preparative method scouting and rapid screening applications. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP Phenyl Wide Pore stationary phase with C3-phenyl bonded chemistry
300 Å pore size for enhanced accessibility of larger molecules
Strong π-π interaction selectivity
Fully double endcapped
High-purity silica support
Reduced silanol activity
Reduced peak tailing
Excellent peak symmetry
Suitable alternative for USP L11 methods
Compatible with HPLC and LC-MS
Typical Applications
USP L11 methods
Positional isomer separations
Aromatic pharmaceutical compounds
Active pharmaceutical ingredient (API) analysis
Peptide separations
Tocopherol analysis
Flavonoid analysis
Polynuclear aromatic compound analysis
Nitro-aromatic compound analysis
Natural product analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl Wide Pore
Chromatography Mode: Reversed Phase
USP Classification: L11
Bonded Phase: C3-Phenyl (Propyl Phenyl)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 0.8-2.0 mL/min
Brand: Cogent
Additional Resources
Special Order Item Information
Important Notes
Suitable as an alternative for USP L11 methods
Often selected when C18 or C8 columns do not provide sufficient selectivity
Excellent for aromatic and structurally similar compounds
Effective for peptides and moderately polar analytes
Wide pore design can improve accessibility for larger molecules
Commonly used in GMP, QC, and research laboratories
Frequently used as a scouting column prior to preparative method development
A Cogent Column Filter is included and recommended to help protect the column and extend service life
Special Order Item