Semi Prep HPLC Column, RP C8 Octyl, Wide Pore, 10um, 300A. 10mm ID x 150mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C8 Octyl Wide Pore is a reversed-phase semi-preparative HPLC stationary phase manufactured using high-purity silica with traditional octyl (C8) silane bonding and complete double endcapping. The 300 Å pore structure is specifically designed to improve accessibility for proteins, peptides, and other large molecules while maintaining the moderate hydrophobicity and selectivity characteristic of C8 chemistry. This stationary phase is well suited for semi-preparative purification workflows where recovery, loading capacity, and efficient fraction collection are critical.
68008-SP150-3M is a semi-preparative HPLC column with dimensions of 10 mm ID x 150 mm length, packed with 10 µm RP C8 Octyl Wide Pore particles featuring a 300 Å pore size. The 10 mm internal diameter provides significantly greater sample loading capacity than analytical columns, while the 150 mm length offers enhanced resolving power for purification of closely related compounds. The 10 µm particle size supports higher flow rates with lower operating backpressure, making this column suitable for purification, method transfer, and scale-up workflows. Typical operating flow rates are approximately 8–15 mL/min depending on sample load, mobile phase composition, and system capabilities.
Designed for purification and fraction collection rather than routine analytical HPLC, this column is particularly useful for proteins, peptides, and other large hydrophobic or neutral compounds. The wide pore architecture improves analyte accessibility while supporting efficient purification of higher molecular weight compounds. Analytical methods are commonly developed on analytical columns before being transferred and optimized on semi-preparative columns such as this one. A semi-preparative HPLC system capable of higher flow rates, larger injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C8 Octyl Wide Pore stationary phase
300 Å pore size optimized for larger molecules
Fully double endcapped chemistry
Traditional octyl (C8) bonded phase
Designed for semi-preparative HPLC purification
Increased sample loading capacity
Excellent for proteins and peptides
10 µm particles for lower operating backpressure
Suitable for higher semi-preparative flow rates
Ideal for purification and fraction collection
Typical Applications
Semi-preparative HPLC purification
Protein purification
Peptide purification
Fraction collection
Biomolecule isolation
Pharmaceutical research
Biotechnology research
Process development
Method scale-up
Production support purification
Specifications
Product Type: Semi-Preparative HPLC Column
Stationary Phase: RP C8 Octyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Octyl (C8)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 10 mm ID x 150 mm
Length: 150 mm
Typical Flow Rate: 8–15 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Intended for purification and fraction collection rather than routine analytical HPLC
Optimized for proteins, peptides, and other high molecular weight compounds
Increased loading capacity compared to analytical columns
Longer column length can improve purification of closely related compounds
Analytical methods are often developed on analytical columns before transfer to semi-preparative columns
Requires a semi-preparative HPLC system capable of higher flow rates and fraction collection
Special Order Item