Prep HPLC Column, RP C8 Octyl, Wide Pore, 10um, 300A. 30mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C8 Octyl Wide Pore is a reversed-phase preparative HPLC stationary phase manufactured using high-purity silica with traditional octyl (C8) silane bonding and complete double endcapping. The 300 Å pore structure is specifically designed to improve accessibility for proteins, peptides, and other large biomolecules while maintaining the moderate hydrophobicity and selectivity characteristic of C8 chemistry. This stationary phase is well suited for preparative purification workflows where high recovery, efficient fraction collection, and loading capacity are critical.
68008-P30-2503M is a preparative HPLC column with dimensions of 30 mm ID x 250 mm length, packed with 10 µm RP C8 Octyl Wide Pore particles featuring a 300 Å pore size. The very large internal diameter provides exceptionally high sample loading capacity for preparative-scale purification, while the 250 mm length delivers maximum resolving power and peak capacity for demanding biomolecule separations. The 10 µm particle size supports higher flow rates with lower operating backpressure, making this column suitable for purification, process development, and scale-up workflows. Typical operating flow rates are approximately 30–80 mL/min depending on sample load, mobile phase composition, and system capabilities.
Designed for purification and fraction collection rather than routine analytical HPLC, this column is particularly useful for proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. The extended column length can significantly improve separation of closely related biomolecules and help achieve higher purity during preparative isolations. Analytical methods are typically developed and optimized on analytical columns before transfer to preparative columns such as this one. A specialized preparative HPLC system capable of higher flow rates, larger injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C8 Octyl Wide Pore stationary phase
300 Å pore size optimized for biomolecules
Fully double endcapped chemistry
Traditional octyl (C8) bonded phase
Designed for preparative HPLC purification
Exceptionally high sample loading capacity
Excellent for proteins and peptides
10 µm particles for lower operating backpressure
Suitable for higher preparative flow rates
Maximum resolution with 250 mm length
Typical Applications
Preparative HPLC purification
Protein purification
Peptide purification
Enzyme purification
Biomolecule isolation
Biopharmaceutical research
Biotechnology research
Process development
Method scale-up
Fraction collection
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: RP C8 Octyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Octyl (C8)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 30 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 30–80 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Intended for purification and fraction collection rather than routine analytical HPLC
Optimized for proteins, peptides, enzymes, and other large biomolecules
Exceptionally high loading capacity supports large-scale preparative isolations
Extended column length can improve separation of closely related biomolecules
Analytical methods are often developed on analytical columns before transfer to preparative columns
Requires a preparative HPLC system capable of higher flow rates and fraction collection
Special Order Item