HPLC Column, RP C8 Octyl, Wide Pore, 10um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C8 Octyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional octyl (C8) silane bonding and complete double endcapping. The 300 Å pore structure is specifically designed to improve accessibility for larger molecules while maintaining the moderate hydrophobicity associated with C8 chemistry. This stationary phase is well suited as an alternative for USP methods that specify L7 column classifications, particularly when larger analytes require enhanced pore accessibility and efficient mass transfer.
68008-25P-4-3M is an analytical HPLC column with dimensions of 4.0 mm ID x 250 mm length, packed with 10 µm RP C8 Octyl Wide Pore particles featuring a 300 Å pore size. The 250 mm length provides maximum resolving power and peak capacity within the analytical RP C8 column family, making it ideal for challenging separations involving closely related compounds. The 10 µm particle size provides lower operating backpressure while supporting reliable and reproducible analytical performance.
This column is well suited for analysis of large hydrophobic or neutral compounds, including proteins, peptides, long-chain fatty acids, and other high-molecular-weight analytes. The wide pore architecture improves analyte accessibility while the moderate retention characteristics of the C8 phase can provide faster elution than C18 columns. The 250 mm format remains a preferred configuration in many established and validated methods used in pharmaceutical, biotechnology, and quality control laboratories. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C8 Octyl Wide Pore stationary phase
300 Å pore size optimized for larger molecules
Fully double endcapped chemistry
Traditional octyl (C8) bonded phase
Suitable alternative for USP L7 methods
Lower retention than C18 columns
10 µm particles for lower operating backpressure
Maximum resolution with 250 mm length
High-purity silica support
Robust analytical performance
Typical Applications
USP L7 methods
Protein analysis
Peptide analysis
Long-chain fatty acid analysis
Large molecule separations
Pharmaceutical analysis
Quality control testing
Biotechnology research
Method validation
Reversed-phase HPLC separations
Specifications
Product Type: Analytical HPLC Column
Stationary Phase: RP C8 Octyl Wide Pore
Chromatography Mode: Reversed Phase
USP Classification: L7
Bonded Phase: Octyl (C8)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Suitable as an alternative for USP L7 methods
Designed for larger molecules requiring wide pore accessibility
Provides less retention than C18 stationary phases
Commonly used for protein, peptide, and long-chain fatty acid separations
Maximum column length provides enhanced resolving power and peak capacity
Frequently specified in validated and legacy analytical methods
Compatible with conventional HPLC systems
Special Order Item