Prep HPLC Column, Diamond Hydride, 4 um, 100 A. 30 mm ID x 250 mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 70000‑P30‑250 Diamond Hydride Preparative HPLC Column is designed for maximum‑resolution, process‑scale purification workflows where shorter preparative columns cannot provide sufficient separation. This full‑length, large‑diameter column delivers the highest resolving power available within the 30 mm ID class while supporting high‑capacity fraction collection for downstream research, development, or commercial use.
Preparative Use Case: Why 30 mm ID × 250 mm
The 30 mm internal diameter defines this column as true process‑scale preparative, intended for laboratories performing large‑volume injections and collecting substantial quantities of purified analytes.
The 250 mm column length provides:
Maximum theoretical plate count within the 30 mm preparative format
Superior resolution of closely eluting or structurally similar compounds
Highest peak capacity for highly complex polar mixtures
Increased confidence during high‑value, large‑scale fraction collection
This configuration is typically selected when resolution is the dominant requirement, and 50 mm, 100 mm, or 150 mm preparative columns cannot adequately resolve target compounds.
Diamond Hydride™ TYPE‑C Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase provides proven advantages for demanding preparative HILIC separations:
Highly stable and reproducible retention of polar and basic analytes
Faster equilibration compared to conventional bonded‑silica HILIC phases
Excellent chemical stability under high‑organic mobile‑phase conditions
Extended column lifetime in continuous or high‑duty‑cycle purification workflows
This chemistry is particularly effective for purification of:
Peptides and peptide‑like compounds
Metabolites and highly polar small molecules
Amine‑containing and basic analytes
Compounds best isolated using HILIC‑based retention mechanisms
Purpose‑Built for Maximum‑Resolution Process Purification
The 70000‑P30‑250 enables predictable scale‑up from smaller preparative formats while preserving chromatographic behavior. It is most often used in method‑critical purification, advanced process development, and regulated or commercial workflows where separation quality cannot be compromised.
System Requirements
Typical operating flow rates: ~35–60 mL/min
This column requires a dedicated preparative or process‑scale HPLC system capable of:
Sustained high‑flow solvent delivery
Very large injection volumes
Pressure limits appropriate for long preparative columns
Suitable detector configuration and fraction collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase composition, solvent viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
These columns represent a significant investment and are frequently application‑specific. To ensure customers receive the correct column for their workflow:
Pricing and technical support are provided through our Customer Service Department via formal quotation
Columns are produced on order, based on customer requirements
Orders cannot be canceled or returned once placed
Learn more about Custom Orders and our Custom Order policies.