Prep HPLC Column, Diamond Hydride, 4 um, 100 A. 21.2 mm ID x 100 mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 70000‑P21‑100 Diamond Hydride Preparative HPLC Column is designed for high‑load preparative purification where both throughput and chromatographic resolution are required. This mid‑length preparative format provides greater resolving power than short 50 mm columns while maintaining efficient cycle times for routine purification workflows.
Preparative Use Case: Why 21.2 mm ID × 100 mm
The 21.2 mm internal diameter defines this column as fully preparative, suitable for laboratories collecting milligram‑ to gram‑scale quantities of purified compounds.
The 100 mm column length provides a balanced performance profile:
Increased resolution compared to 50 mm preparative columns
Improved separation of partially co‑eluting components
Moderate backpressure at high preparative flow rates
Strong balance between separation quality and throughput
This configuration is typically selected when short prep columns lack sufficient resolution, but full‑length 150–250 mm columns are unnecessary or inefficient for the application.
Diamond Hydride™ TYPE‑C Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase delivers proven performance advantages for preparative HILIC separations:
Highly reproducible retention of polar analytes
Faster equilibration relative to conventional bonded‑silica HILIC phases
Robust chemical and mechanical stability under high‑organic mobile phases
Long column lifetime in demanding preparative environments
This chemistry is especially effective for purification of:
Peptides and peptide‑like compounds
Metabolites and other highly polar molecules
Amine‑containing and basic analytes
Compounds best isolated using HILIC‑based retention mechanisms
Purpose‑Built for Resolution‑Focused Prep Work
The 70000‑P21‑100 supports predictable scale‑up from analytical and semi‑preparative HILIC methods while preserving chromatographic behavior. It is frequently used in method‑critical purification, process development, and regulated workflows where resolution cannot be sacrificed, but maximum column length is not required.
System Requirements
Typical operating flow rates: ~20–40 mL/min
This column requires a preparative HPLC system capable of:
Sustained high‑flow solvent delivery
Large injection volumes
Pressure limits appropriate for preparative column hardware
Compatible detector configurations and fraction collection
Standard analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates may be optimized based on mobile‑phase composition, viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
These columns represent a significant investment and are often application‑specific. To ensure customers receive the correct column for their workflow:
Pricing and technical support are provided through our Customer Service Department via formal quotation
Columns are produced on order, based on customer requirements
Orders cannot be canceled or returned once placed
Learn more about Custom Orders and our Custom Order policies.