HPLC Column, Amide 2.o, 2.2 um, 3.0 mm ID x 100 mm Length, 120 A. Cogent TYPE-C Brand. 1 EA. Special Order Item.
Additional Information:
High-Resolution Amide HILIC Column for Demanding Polar Analyte Separations
The 40236-10P-3 Amide HPLC Column is manufactured using Cogent TYPE-C™ silica hydride technology and is designed for HILIC applications that require increased retention, enhanced resolution, and excellent reproducibility. The amide stationary phase provides strong retention of highly polar compounds while the silica hydride support promotes stable chromatographic behavior and reliable analytical performance.
Combining advanced surface chemistry with 2.2 µm high-efficiency particles, this column is well suited for laboratories analyzing difficult polar compounds that are not adequately retained by conventional reversed-phase methods. The longer 100 mm length increases resolving power, making it ideal for complex samples and closely eluting analytes.
Optimized for HILIC Analysis of Polar Compounds
The Amide stationary phase is commonly selected for compounds containing hydroxyl, amine, and other polar functional groups. Typical applications include:
Carbohydrates and sugars
Sugar alcohols
Amino-containing compounds
Polar metabolites
Pharmaceutical compounds
Food and beverage ingredients
Biological and biochemical analytes
Other highly polar compounds requiring strong HILIC retention
The unique TYPE-C™ silica hydride platform helps maintain consistent retention characteristics and robust performance across a wide range of HILIC conditions.
Advantages of Cogent TYPE-C™ Amide Technology
Compared with many traditional silica-based amide phases, this technology offers:
Fast equilibration between analyses
Excellent retention reproducibility
Stable operation under high-organic mobile-phase conditions
Reliable retention of highly polar compounds
Reduced variability associated with conventional water-rich surface layers
Long-term stationary-phase durability
These benefits support both routine analytical testing and complex method-development projects.
Why Choose the 3.0 mm ID × 100 mm Configuration
The 3.0 mm internal diameter format provides an excellent balance between chromatographic performance and solvent efficiency. Compared with conventional 4.6 mm ID columns, it significantly reduces solvent consumption while maintaining strong analytical capability.
Benefits include:
Enhanced chromatographic resolution
Increased peak capacity
Reduced mobile-phase consumption
Improved laboratory efficiency
Excellent compatibility with LC-MS and LC-MS/MS methods
Strong performance for routine and research applications
The 100 mm length provides additional theoretical plates compared to shorter columns, making it ideal when separation quality is more important than maximum speed.
Small Particle Technology for Improved Performance
The 2.2 µm particle size provides high chromatographic efficiency, improved peak shape, and enhanced sensitivity. Combined with the longer column length, this format delivers excellent resolving power for complex HILIC separations while maintaining practical analysis times.
Analysts frequently select this configuration when additional separation performance is needed beyond what shorter columns can provide.
System Requirements and Flow Rates
The 3.0 mm ID format offers a useful balance between sensitivity and ease of operation.
Typical flow-rate range (3.0 mm ID): approximately 0.4-0.6 mL/min
Suitable for modern HPLC and UHPLC systems
Excellent choice for LC-MS workflows utilizing reduced solvent volumes
Longer column length may generate higher operating pressures than shorter column configurations
Method conditions should be optimized according to application requirements, instrument capabilities, and mobile-phase composition
When to Select a 100 mm Amide Column
A 100 mm column length is often beneficial when methods require:
Improved separation of closely related compounds
Greater chromatographic resolution
Increased peak capacity
Enhanced retention of difficult polar analytes
Improved performance in complex sample matrices
Greater confidence in qualitative and quantitative analyses
This configuration provides a strong balance between throughput and analytical performance for HILIC method development and routine testing.
Column Specifications
Stationary Phase: Amide
Support Technology: Cogent TYPE-C™ Silica Hydride
Particle Size: 2.2 µm
Pore Size: 120 Å
Internal Diameter: 3.0 mm
Length: 100 mm
Separation Mode: HILIC
Special Order Item Information
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