HPLC Column, Amide 2.o, 2.2 um, 3.0 mm ID x 50 mm Length, 120 A. Cogent TYPE-C Brand. 1 EA. Special Order Item.
Additional Information:
High-Efficiency Amide HILIC Column for Polar Compound Retention
The 40236-05P-3 Amide HPLC Column is based on Cogent TYPE-C™ silica hydride technology and is engineered for challenging HILIC separations involving highly polar compounds. The amide stationary phase provides strong and reproducible retention of analytes that are often difficult to retain by reversed-phase chromatography.
By combining advanced silica hydride support material with 2.2 µm high-efficiency particles, this column delivers excellent chromatographic performance, fast equilibration, and robust method reproducibility. It is well suited for laboratories seeking improved throughput while maintaining the selectivity advantages of amide HILIC chemistry.
Ideal for Polar Compound Analysis
The Amide stationary phase is particularly effective for compounds possessing hydroxyl, amine, and other highly polar functional groups. Typical applications include:
Carbohydrates and sugars
Sugar alcohols
Amino-containing compounds
Polar pharmaceutical compounds
Metabolites and metabolic intermediates
Food and beverage analytes
Other HILIC-retained compounds
The TYPE-C™ silica hydride platform helps provide stable retention behavior and dependable analytical performance across a broad range of HILIC method conditions.
Benefits of Cogent TYPE-C™ Amide Technology
This advanced stationary phase offers several advantages over conventional silica-based amide HILIC columns:
Fast column equilibration
Excellent run-to-run reproducibility
Reliable retention of highly polar analytes
Robust operation under high-organic mobile-phase conditions
Reduced variability associated with excess water-layer formation
Long stationary-phase lifetime under routine laboratory use
These characteristics make the column suitable for both routine analyses and demanding method-development projects.
Why Choose the 3.0 mm ID × 50 mm Configuration
The 3.0 mm internal diameter format provides an excellent balance between analytical sensitivity, throughput, and solvent savings. Compared to traditional 4.6 mm ID methods, this configuration helps reduce mobile-phase consumption while maintaining strong chromatographic performance. Benefits include:
Fast analysis times
Reduced solvent consumption
Improved laboratory efficiency
Excellent compatibility with LC-MS and LC-MS/MS workflows
Increased sample throughput
Rapid column equilibration between injections
The 50 mm column length is particularly useful when speed and productivity are primary analytical objectives.
Small Particle Performance
The 2.2 µm particle size provides high column efficiency and excellent peak shape, allowing analysts to achieve rapid separations with enhanced resolution and sensitivity. The result is improved productivity without sacrificing analytical performance.
System Requirements and Flow Rates
The 3.0 mm ID format is often selected as a solvent-saving alternative to larger analytical columns while remaining easier to operate than narrower 2.1 mm columns.
Typical flow-rate range (3.0 mm ID): approximately 0.4-0.6 mL/min
Suitable for UHPLC and modern HPLC systems capable of handling higher-efficiency columns
Excellent choice for LC-MS applications requiring reduced solvent loads
Method conditions should be optimized according to mobile-phase composition, operating pressure, and application requirements
HILIC Method Development Considerations
For optimal HILIC performance, mobile phases commonly employ elevated organic solvent content with controlled aqueous composition. The Amide stationary phase is particularly useful when stronger retention of highly polar analytes is desired while maintaining excellent peak shape and method reproducibility.
Special Order Item Information
Special Order Item Information: See the MICROSOLV Special Order Item Information page for ordering requirements, lead-time details, and applicable return policies.
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