HPLC Column, Amide 2.o, 2.2 um, 2.1 mm ID x 150 mm Length, 120 A. Cogent TYPE-C Brand. 1 EA.
Additional Information:
Maximum Resolution Amide HILIC Column for Complex Polar Compound Analysis
The 40236-15P-2 Amide HPLC Column is manufactured using Cogent TYPE-C™ silica hydride technology and is designed for demanding HILIC applications requiring exceptional retention, high peak capacity, and superior chromatographic resolution. The amide stationary phase provides strong retention of highly polar analytes, making it an excellent choice when conventional reversed-phase methods do not provide adequate retention or separation.
Combining advanced silica hydride technology with 2.2 µm high-efficiency particles, this column delivers excellent reproducibility, robust retention characteristics, and outstanding separation performance. The 150 mm length provides the highest resolving power within this product family and is particularly valuable for complex sample matrices and difficult separations.
Optimized for HILIC Retention of Polar Analytes
The Amide stationary phase is especially effective for analytes containing hydroxyl, amine, and other strongly polar functional groups. Typical applications include:
Carbohydrates and sugars
Sugar alcohols
Amino-containing compounds
Polar pharmaceutical compounds
Metabolites and metabolic intermediates
Food and beverage analytes
Biological and biochemical compounds
Other highly polar compounds requiring HILIC retention
The unique TYPE-C™ silica hydride support helps provide stable retention behavior and excellent method reproducibility across a broad range of HILIC conditions.
Advantages of Cogent TYPE-C™ Amide Technology
Compared to many traditional silica-based HILIC columns, this stationary phase offers:
Fast equilibration between injections
Excellent batch-to-batch and run-to-run reproducibility
Consistent retention of highly polar compounds
Stable operation in high-organic mobile phases
Reduced variability associated with conventional water-rich surface layers
Durable, long-lasting chromatographic performance
These advantages simplify method development while supporting reliable routine analytical workflows.
Why Choose the 2.1 mm ID × 150 mm Configuration
The 2.1 mm internal diameter format is widely used for LC-MS and UHPLC applications where high sensitivity and reduced solvent consumption are important. The 150 mm length provides substantially greater resolving power than shorter columns, making it an excellent choice when analytical quality is prioritized over maximum speed.
Benefits include:
Maximum chromatographic resolution
Increased peak capacity
Enhanced separation of closely related compounds
Reduced solvent consumption
Excellent compatibility with LC-MS and LC-MS/MS systems
Improved confidence in complex analyses
Greater retention for difficult polar analytes
This column is often selected when shorter columns do not provide sufficient separation for critical analytical methods.
Small Particle Technology for Superior Efficiency
The 2.2 µm particle size produces highly efficient separations with narrow peak widths and excellent peak symmetry. When combined with the 150 mm column length, this particle size provides exceptional chromatographic performance for complex HILIC applications.
Analysts can achieve improved quantitative accuracy, enhanced sensitivity, and greater confidence in identifying closely eluting compounds.
System Requirements and Flow Rates
The 2.1 mm ID format is optimized for low-flow analytical methods and LC-MS applications.
Typical flow-rate range (2.1 mm ID): approximately 0.2-0.3 mL/min
Requires an HPLC or UHPLC system capable of stable and accurate low-flow solvent delivery
Well suited for LC-MS and LC-MS/MS workflows
Longer column length may generate higher backpressure than 50 mm or 100 mm configurations
Actual operating conditions should be optimized according to mobile-phase composition, temperature, and instrument limitations
Not all conventional HPLC systems are capable of delivering optimal performance at the low flow rates commonly used with 2.1 mm ID columns.
When to Select a 150 mm Column Length
A 150 mm column is often the preferred choice when methods require:
Maximum chromatographic resolution
Greater peak capacity
Separation of structurally similar compounds
Enhanced performance with complex sample matrices
Improved retention of highly polar analytes
Greater confidence in qualitative and quantitative analyses
Advanced HILIC method development
While shorter columns are frequently selected for speed, the 150 mm format provides the highest level of separation performance for challenging analytical applications.
Column Specifications
Stationary Phase: Amide
Support Technology: Cogent TYPE-C™ Silica Hydride
Particle Size: 2.2 µm
Pore Size: 120 Å
Internal Diameter: 2.1 mm
Length: 150 mm
Separation Mode: HILIC
Availability Information