Cogent RP Biphenyl, 5 um, 4.6 mm ID x 250 mm Length, 100 A. Cogent Brand. 1 EA.
Additional Info:
Enhance Selectivity for Aromatic Compounds with Cogent RP Biphenyl HPLC Columns!
68552‑25P provides enhanced aromatic selectivity using biphenyl functionality that enables strong π‑π interactions, offering improved resolution of structurally similar compounds that are difficult to separate with traditional C18 phases. The extended 250 mm column length increases theoretical plates, delivering higher resolution for complex separations and impurity profiling.
With 5 µm particles and a 100 Å pore size, this column offers robust performance for demanding analytical methods, maintaining excellent peak shape, reproducibility, and durability while enabling deeper separation of closely related analytes.
Why Choose RP Biphenyl?
Enhanced Aromatic Selectivity Ideal for compounds containing phenyl groups or conjugated systems, providing improved resolution of positional isomers and structurally similar impurities.
Improved Separation of Challenging Compounds Offers different retention mechanisms than C18, enabling better separation of polar aromatics, halogenated compounds, and drug metabolites.
Key Benefits
π‑π interaction selectivity ✅ Better resolution of aromatic compounds
Reversed‑phase versatility ✅ Suitable for a wide range of analytes
5 µm particle size ✅ Robust, reproducible performance for routine methods
100 Å pore size ✅ Ideal for small molecule analysis
Standard 4.6 mm ID format ✅ Widely compatible with HPLC systems
250 mm length ✅ Maximum resolution for complex separations
Typical Applications
Pharmaceutical Analysis Accurate quantification of APIs, degradants, and impurities, especially aromatic drug compounds
Environmental Testing Detection of pesticides, phenols, and other aromatic pollutants in water and soil
Food Safety Analysis of additives, preservatives, and contaminants with aromatic structures
General Analytical Chemistry Separation of structurally similar compounds where C18 selectivity is insufficient
HPLC Column Use‑Case Framework
250 mm Length ✅ Maximum resolution and peak capacity
Ideal for complex separations and impurity profiling
Increased run time compared to shorter columns
4.6 mm ID (Standard Analytical) ✅
Typical flow rate: ~0.8–1.2 mL/min
Ideal for routine HPLC systems
Robust and widely compatible format for method development and QC
Flow rate may be adjusted based on mobile phase viscosity, temperature, and system pressure limits.
Important Notes
Designed for reversed‑phase HPLC methods
Provides alternative selectivity vs traditional C18 phases
Longer column length increases resolution but may increase run time
Compatible with standard HPLC instrumentation
Suitable for method development and high‑resolution workflows