Guard Column cartridge, UDC Cholesterol 2.o, replacement, 2.0 mm ID x 10 mm long cartridge packed with 2.2 um, 120 A phase. Cogent TYPE-C Brand. 1 EA.

Additional Info:

The 69269‑PG3 Cogent UDC‑Cholesterol 2.0 Guard Column Cartridge is a single replacement cartridge designed for UHPLC and high‑pressure analytical systems, providing essential protection while preserving shape‑selective chromatographic performance.

Each cartridge is:

  • 2.0 mm ID × 10 mm length

  • Packed with 2.2 µm, 120 Å TYPE‑C™ silica hydride UDC‑Cholesterol 2.0 phase

  • Constructed in stainless steel housing with SS frits

The cartridge is supplied in an individual protective Delrin™ case, preventing contamination, drying, or physical damage prior to use.

⚠️ Requires guard column holder (sold separately)

Designed for 2.1 mm UHPLC Columns

This cartridge is optimized for:

  • 2.1 mm ID UHPLC and analytical columns

  • Sub‑2 µm particle methods

  • LC‑MS workflows requiring high sensitivity

Proper guard column matching ensures:

  • Minimal band broadening despite small particle size

  • Maintained high efficiency and resolution

  • Consistent retention and selectivity

Why Use a Single Guard Cartridge

A single‑cartridge format is ideal when:

  • Evaluating different guard phases in method development

  • Using multiple analytical column chemistries

  • Replacing cartridges individually as needed

  • Maintaining flexibility without committing to multi‑packs

This provides maximum control and adaptability in UHPLC workflows.

Why Use UHPLC Guard Column Protection

Columns packed with 2.2 µm particles are highly sensitive to:

  • Particulate contamination

  • Strongly retained analytes

  • Sample matrix buildup

  • Pressure increases due to partial clogging

The 69269‑PG3 cartridge acts as a critical sacrificial protection layer, helping to:

  • Preserve UHPLC column efficiency and resolution

  • Prevent rapid backpressure increases

  • Extend column lifetime

  • Maintain consistent analytical performance

Cartridges can be replaced quickly without disturbing the analytical column.

UDC‑Cholesterol™ 2.0 Guard Phase Advantages

Matching the guard phase to the analytical column ensures:

  • Identical shape selectivity and retention behavior

  • No need for method re‑optimization

  • Seamless analyte transfer

Shape‑Selective Interaction

  • Separates compounds based on 3D molecular structure and steric fit

  • Ideal for isomers and structurally similar analytes

Strong Hydrophobic Retention

  • Effective for lipids and hydrophobic compounds

Tunable Selectivity

  • Adjust separations using:

    • Temperature changes

    • Mobile phase composition

Cogent TYPE‑C™ Technology Benefits

Silica Hydride Surface

  • Direct Si–C bonding (no end‑capping required)

  • Reduced silanol activity

  • Improved peak shape for basic and polar analytes

High‑Pressure Stability

  • Designed for UHPLC / sub‑2 µm applications

  • Compatible with elevated system pressures

  • Maintains performance under demanding conditions

Faster Equilibration

  • Reduced cycle times

  • Increased analytical throughput

Typical Applications

  • UHPLC and LC‑MS analytical workflows

  • Steroidal compound analysis

  • Structural isomer separations

  • Lipids and hydrophobic analytes

  • Peptides, proteins, and oligonucleotides

  • Methods requiring shape‑selectivity preservation

 

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