Guard Column Starter Set, UDC Cholesterol 2.o, complete with 5 each 2.0 mm ID x 10 mm long cartridges packed with 2.2 um, 120 A phase and 1 each guard column holder. Cogent TYPE-C Brand. 1 EA.

Additional Info:

The 69269‑PG1 Cogent UDC‑Cholesterol 2.0 Guard Column Starter Set is a complete high‑pressure protection system designed for UHPLC and LC‑MS workflows, where maintaining column efficiency and shape‑selective performance is critical.

This starter kit includes:

Each cartridge is individually packaged in a protective Delrin™ case, preventing contamination, drying, or damage before use.

Designed for 2.1 mm UHPLC Columns

This guard column system is optimized for:

  • 2.1 mm ID UHPLC and analytical columns

  • Sub‑2 µm particle methods

  • LC‑MS workflows requiring high sensitivity

Proper guard column integration ensures:

  • Minimal band broadening

  • Maintained high efficiency and resolution

  • Consistent retention and selectivity

Why Use a UHPLC Guard Column System

UHPLC columns using 2.2 µm particles are highly sensitive to:

  • Particulates and contamination

  • Strongly retained analytes

  • Sample matrix buildup

  • Pressure increases due to blockages

The 69269‑PG1 starter kit provides a critical sacrificial protection layer, helping to:

  • Preserve column efficiency and resolution

  • Prevent rapid pressure buildup

  • Extend UHPLC column lifetime

  • Maintain reproducibility in high‑performance methods

In typical use, the five included cartridges often protect an analytical column for its usable lifetime, depending on sample cleanliness.

UDC‑Cholesterol™ 2.0 Guard Phase Advantages

Using a matched guard phase ensures:

  • Identical shape selectivity and retention behavior

  • No method disruption

  • Seamless analyte transfer to the analytical column

Shape‑Selective Interaction

  • Separates compounds based on 3D structure and steric fit

  • Ideal for isomers and structurally similar analytes

Strong Hydrophobic Interaction

  • Effective retention of lipids and hydrophobic compounds

Tunable Selectivity

  • Optimize separations using:

    • Temperature adjustments

    • Mobile phase composition

Cogent TYPE‑C™ Technology Benefits

Silica Hydride Surface

  • Direct Si–C bonding (no end‑capping required)

  • Reduced silanol interactions

  • Improved peak shape for basic and polar analytes

High‑Pressure Stability

  • Designed for UHPLC / sub‑2 µm workflows

  • Compatible with elevated system pressures

  • Maintains performance under demanding conditions

Faster Equilibration

  • Reduced cycle times

  • Increased throughput for high‑efficiency methods

Typical Applications

  • UHPLC and LC‑MS analytical workflows

  • Steroidal compound analysis

  • Structural isomer separations

  • Lipids and hydrophobic analytes

  • Peptides, proteins, and oligonucleotides

  • Methods requiring shape‑selectivity preservation

 

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