Catalog Number - 70200-15P-3

High‑Efficiency Diamond Hydride™ 2.o Performance in a Solvent‑Saver Format

The 70200‑15P‑3 Diamond Hydride™ 2.o HPLC Column is a custom‑manufactured analytical column engineered to deliver high efficiency and fast equilibration while reducing solvent consumption and operating pressure. Built on Cogent TYPE‑C™ silica hydride technology with a 2.2 µm particle size, this column provides near‑UHPLC performance using conventional HPLC systems.

The 3.0 mm ID × 150 mm length format occupies a highly effective middle ground between traditional 4.6 mm analytical columns and narrow‑bore 2.1 mm LC‑MS columns—offering improved efficiency and solvent savings without the stringent low‑flow requirements of narrower formats.

Why the 3.0 mm × 150 mm Configuration Is Selected

This column format is intentionally chosen for laboratories seeking improved performance with minimal system constraints:

• Substantially reduced solvent consumption compared to 4.6 mm ID columns

• Comparable run times to standard analytical columns at lower flow rates

• Lower backpressure while maintaining high plate count

• Straightforward compatibility with conventional HPLC instrumentation

For many laboratories, the 3.0 mm ID configuration serves as an ideal “solvent‑saver” analytical column that improves efficiency without requiring UHPLC hardware or extreme low‑flow capability.

Diamond Hydride™ 2.o Stationary Phase Advantage

The Diamond Hydride™ 2.o stationary phase is manufactured using Cogent TYPE‑C™ silica hydride technology with smaller 2.2 µm particles, providing advantages over both traditional HILIC phases and larger‑particle hydride materials:

• Faster equilibration between injections

• Increased column efficiency and sharper peak shapes

• Stable and reproducible retention at high organic content

• Mixed‑mode interaction capability for challenging polar analytes

These features enable high‑resolution separations with excellent robustness under routine analytical conditions.

Ideal Applications

The 70200‑15P‑3 Diamond Hydride 2.o column is routinely used for:

• Small, highly polar molecules

• Amino acids and organic acids

• Polar and moderately non‑polar peptides

• Metabolites and other biologically relevant compounds

It is especially well‑suited for laboratories transitioning toward higher efficiency methods without converting fully to UHPLC platforms.

High‑Resolution HPLC and UV Detection

The 150 mm column length provides increased plate count and resolving power, making this column effective for:

• Analytical methods requiring improved resolution over shorter columns

• Routine and validated workflows where reproducibility and robustness are critical

• UV‑based detection using standard flow cells and detector configurations

Unlike narrow‑bore columns, the 3.0 mm ID format maintains comfortable injection volumes and detector compatibility.

LC‑MS Compatibility

While optimized for conventional HPLC workflows, the 70200‑15P‑3 is fully LC‑MS compatible where moderate analytical flow rates are acceptable. It is commonly selected for LC‑MS methods when solvent reduction and efficiency gains are desired without adopting 2.1 mm low‑flow constraints.

Operating Guidelines and System Requirements

• Typical flow‑rate range: ~0.4–0.6 mL/min (method‑ and solvent‑dependent)

• System compatibility: All standard analytical HPLC systems

• Special requirements: No specialized low‑flow capability required

Flow rates may be optimized further based on resolution targets, pressure limits, and detector configuration.