Catalog Number - 59501-25P

MicroBee Silica™ is an unbonded, Type-A silica HPLC stationary phase designed for normal phase separations using traditional adsorption chromatography mechanisms. As part of the Cogent™ Similars product line, it has been a trusted value-oriented choice for laboratories running established silica methods for many years. The untreated silica surface provides strong interactions with polar analytes while offering broad applicability for normal phase chromatography and legacy method replacement. Unmodified silica is also suitable for HILIC applications when appropriate mobile phase conditions are used.

59501-25P is a 250 mm x 4.6 mm ID analytical column packed with 5 µm, 125Å MicroBee Silica particles. The 250 mm length provides increased resolving power compared to shorter columns, making it well suited for complex separations and methods requiring enhanced chromatographic performance. The 5 µm particle size delivers greater efficiency and sharper peak shapes than 10 µm packings, although higher operating backpressure should be expected. The industry-standard 4.6 mm ID format typically operates optimally at approximately 1.0–2.0 mL/min on conventional HPLC systems.

Because the stationary phase consists of completely unmodified silica, this USP L3 column is an excellent alternative for legacy methods developed on Porasil® Silica and similar columns. It is suitable for separating hydrophilic, hydrophobic, polar, and non-polar compounds under normal phase conditions while also serving as a versatile option for HILIC method development and routine analytical applications.

Key Benefits

• Unmodified Type-A silica stationary phase

• Suitable alternative for USP L3 method requirements

• Higher efficiency with 5 µm particles

• Increased resolution from 250 mm column length

• Ideal for normal phase chromatography

• Suitable for HILIC applications

• Comparable to traditional Porasil® Silica columns

• Cost-effective Cogent Similars column

Typical Applications

• USP L3 methods

• Normal phase HPLC

• HILIC separations

• Pharmaceutical analysis

• Method development

• Quality control testing

• Polar compound separations

• Legacy silica column replacement

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Silica

• USP Classification: L3

• Particle Size: 5 µm

• Particle Type: Type-A Silica

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for normal phase chromatography

• Suitable for HILIC methods with appropriate mobile phases

• Unmodified silica stationary phase

• Industry-standard 4.6 mm ID analytical format

• Compatible with conventional HPLC systems

• 5 µm particles provide higher efficiency and improved resolution

• Performance comparable to traditional Porasil® Silica columns

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