Catalog Number - 84518-25P

The 84518‑25P Cogent RP C18 PE™ HPLC Column is engineered for maximum‑resolution analytical separations and highest‑purity results, combining 5 µm particles with a polar embedded amide group within a C18 ligand to deliver robust polar retention and consistent chromatographic performance across a wide range of analytes.

Manufactured using high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous mobile phase conditions, including 100% water

The embedded amide functionality enhances interaction with polar compounds while preventing phase collapse common with conventional C18 phases, maintaining strong reversed‑phase behavior for hydrophobic compounds.

✅ One of the most popular Polar Embedded C18 columns for demanding analytical workflows and USP method alternatives

250 mm Length – Maximum Separation & Highest Purity

• Highest resolving power in the analytical PE column range

• Maximizes peak capacity for highly complex mixtures

• Ideal for closely related compounds and challenging separations

• Provides extended retention and improved separation of difficult analytes

• Best suited for final method development, validation, and highest‑purity analytical results

This configuration is selected when shorter columns cannot achieve required resolution or purity.

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • quality control (QC)

  • regulated laboratory workflows

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Suitable for standard analytical systems without specialized requirements

5 µm Particle – Rugged & Reproducible Performance

• Designed for durability and long column life

• Provides reliable and consistent analytical performance

• Ideal for method transfer and reproducibility across systems and laboratories

✅ Preferred when robustness and consistency are prioritized over maximum efficiency

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar analytes without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Operates in:

  • Reversed Phase (RP)

  • HILIC‑like mechanisms under appropriate conditions

Ideal for Challenging Samples

• Suitable for:

  • polar compounds poorly retained on conventional C18 phases

  • mixed polarity sample matrices

• Enables robust and flexible method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • consistent retention of polar analytes

Typical Applications

• Analytical method development

• Pharmaceutical QC and validation

• Polar and hydrophilic compound separations

• Complex mixture analysis

• Long‑chain fatty acids

• Final method validation workflows

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Maintains classic C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable batch‑to‑batch reproducibility

Important Usage Notes

• Designed for maximum‑resolution analytical workflows

• Suitable for high aqueous mobile phases (including 100% water)

• Compatible with standard 4.6 mm analytical HPLC systems

• Optimize for resolution vs throughput based on method requirements