Catalog Number - 84518-15P-2

The 84518‑15P‑2 Cogent RP C18 PE™ HPLC Column is engineered for maximum‑resolution, low‑flow analytical and LC‑MS workflows, utilizing 5 µm particles with a polar embedded amide group within a C18 ligand to deliver stable polar retention and highly reproducible chromatographic performance.

Manufactured using high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous mobile phase conditions, including 100% water

The embedded amide functionality enhances interaction with polar compounds, preventing phase collapse common to traditional C18 columns, while maintaining strong reversed‑phase selectivity for hydrophobic analytes.

150 mm Length – Maximum Resolution for LC‑MS Applications

• Highest resolving power within the 2.1 mm PE column family

• Maximizes peak capacity for complex mixtures

• Ideal for closely related compounds and difficult separations

• Preferred for final method optimization and high‑resolution LC‑MS workflows

• Longer analysis times compared to shorter columns

This configuration is selected when maximum separation performance is required beyond 50 mm and 100 mm formats.

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Designed for low‑flow HPLC and LC‑MS systems

• Enhances mass spectrometry sensitivity due to reduced solvent load

• Ideal for:

  • trace‑level detection

  • high‑sensitivity analytical workflows

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires precise, stable low‑flow pump capability and low‑volume detection systems

5 µm Particle – Robust & Reproducible Performance

• Designed for long column life and durability in demanding workflows

• Provides consistent performance across runs and systems

• Ideal for method transfer and reproducibility in regulated environments

✅ Preferred when robustness and method stability are prioritized over maximum efficiency

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable under 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Operates in:

  • Reversed Phase (RP)

  • HILIC‑like mechanisms under appropriate conditions

Ideal for Complex Samples

• Suitable for:

  • polar analytes poorly retained on conventional C18

  • mixed polarity sample sets

• Enables robust and flexible method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • consistent retention of polar analytes

Typical Applications

• LC‑MS analysis

• High‑resolution method development

• Polar and hydrophilic compound separations

• Pharmaceutical and QC workflows

• Peptides and small molecules

• Long‑chain fatty acids

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Maintains classical C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable batch‑to‑batch reproducibility

Important Usage Notes

• Designed for 2.1 mm LC‑MS and low‑flow analytical systems

• Requires precise pump control and low‑volume flow cell/detector compatibility

• Ideal for maximum‑resolution LC‑MS workflows

• Suitable for high aqueous mobile phases (including 100% water)

• Optimize based on RP or mixed‑mode separation behavior