Catalog Number - 84518-15P

The 84518‑15P Cogent RP C18 PE™ HPLC Column is engineered for high‑resolution analytical workflows, combining 5 µm particles with a polar embedded amide group within a C18 ligand to deliver enhanced retention of polar analytes alongside reliable hydrophobic selectivity.

Manufactured on high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous mobile phases, including 100% water

The embedded amide functionality prevents phase collapse associated with traditional C18 columns, enabling consistent retention of polar compounds while maintaining rugged reversed‑phase performance.

✅ One of our most popular Polar Embedded C18 Columns for analytical workflows

150 mm Length – Maximum Resolution for Analytical Methods

• Higher resolving power than 50 mm and 100 mm columns

• Maximizes peak capacity for complex mixtures

• Ideal for closely related compounds and challenging separations

• Preferred for final method development and validation workflows

• Longer run times compared to shorter columns

This configuration is selected when maximum separation performance is required before final method deployment.

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • quality control (QC)

  • routine and regulated environments

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Suitable for standard analytical systems without special flow requirements

5 µm Particle – Rugged & Reproducible Performance

• Designed for durability and long column lifetime

• Provides reliable performance across routine analytical workflows

• Ideal for method transfer and reproducibility across laboratories

✅ Preferred when robustness and stability are prioritized over maximum efficiency

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Operates in:

  • Reversed Phase (RP)

  • HILIC‑like behavior under appropriate conditions

Ideal for Challenging Samples

• Suitable for:

  • polar analytes poorly retained on conventional C18

  • mixed polarity sample matrices

• Enables flexible and robust method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • reliable retention of polar analytes

Typical Applications

• Analytical method development

• Pharmaceutical QC and validation

• Polar and hydrophilic compound separations

• Complex mixture analysis

• Long‑chain fatty acids

• Final method validation workflows

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Maintains traditional C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable run‑to‑run reproducibility

Important Usage Notes

• Designed for maximum‑resolution analytical applications

• Suitable for high aqueous mobile phases (including 100% water)

• Compatible with standard 4.6 mm analytical HPLC systems

• Optimize based on RP or mixed‑mode method conditions