Catalog Number - 84518-10P-2

The 84518‑10P‑2 Cogent RP C18 PE™ HPLC Column is designed for robust, high‑performance LC‑MS separations, combining 5 µm particles with a polar embedded amide group within a C18 ligand to deliver enhanced polar retention and reproducible chromatographic performance.

Manufactured using high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous mobile phase conditions, including 100% water

The embedded amide functionality prevents phase collapse common in traditional C18 columns, improving retention of polar analytes while maintaining strong hydrophobic selectivity.

100 mm Length – Increased Resolution for LC‑MS

• Higher resolving power than 50 mm columns

• Improved separation of closely related compounds

• Increased peak capacity for analytical workflows

• Ideal for LC‑MS methods requiring enhanced resolution and peak clarity

This configuration is selected when greater separation performance is needed beyond fast screening methods.

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Designed for low‑flow HPLC and LC‑MS systems

• Enhances MS sensitivity due to reduced solvent load

• Ideal for:

  • trace‑level analysis

  • high‑sensitivity detection workflows

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires HPLC or UHPLC systems capable of stable, precise low‑flow delivery

5 µm Particle – Robust & Reliable Performance

• Designed for durability and long service life

• Provides consistent performance across routine and high‑throughput workflows

• Ideal for method transfer and reproducibility across systems

✅ Preferred when robustness and method stability outweigh maximum efficiency

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Operates in:

  • Reversed Phase (RP)

  • HILIC‑like mechanisms under appropriate conditions

Ideal for Mixed Polarity Samples

• Suitable for:

  • polar analytes with poor retention on conventional C18

  • complex mixtures with wide polarity ranges

• Enables flexible and reliable method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phase conditions

  • consistent retention of polar analytes

Typical Applications

• LC‑MS analysis

• Method development and refinement

• Polar and hydrophilic compound separations

• Pharmaceutical and QC workflows

• Long‑chain fatty acids

• Mixed polarity compound analysis

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Maintains traditional C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable batch‑to‑batch reproducibility

Important Usage Notes

• Designed for 2.1 mm LC‑MS and low‑flow analytical systems

• Requires precise low‑flow pump capability and low‑volume detection systems

• Ideal for increased‑resolution LC‑MS workflows

• Suitable for high aqueous mobile phases (including 100% water)