Catalog Number - 84518-05P-2

The 84518‑05P‑2 Cogent RP C18 PE™ HPLC Column is designed for robust, fast LC‑MS separations, utilizing 5 µm particles with a polar embedded amide functional group within a C18 ligand to deliver stable polar retention and consistent chromatographic performance.

Manufactured on high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous mobile phase conditions (including 100% water)

The embedded amide group enhances interaction with polar compounds, preventing phase collapse seen in conventional C18 columns, while maintaining strong reversed‑phase retention for hydrophobic analytes.

50 mm Length – Fast LC‑MS & High‑Throughput Analysis

• Designed for rapid separations and short run times

• Ideal for high‑throughput analytical workflows

• Reduces solvent consumption

• Commonly used for LC‑MS screening and routine analysis

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Optimized for low‑flow HPLC and LC‑MS systems

• Enhances mass spectrometer sensitivity due to reduced solvent load

• Ideal for:

  • trace‑level detection

  • high‑sensitivity analytical methods

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires HPLC/UHPLC systems capable of stable low‑flow delivery

5 µm Particle – Robust & Reliable Performance

• Designed for durability and long column lifetime

• Provides reliable performance in routine analytical workflows

• Ideal for method development and high‑throughput applications

✅ Preferred when robustness and method transferability are prioritized over maximum efficiency

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Can function in:

  • Reversed Phase (RP)

  • HILIC‑like behavior under appropriate conditions

Ideal for Mixed Polarity Samples

• Suitable for:

  • polar analytes poorly retained on conventional C18

  • complex mixtures with wide polarity range

• Enables flexible method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • consistent retention of polar analytes

Typical Applications

• LC‑MS analysis

• Fast screening and method development

• Polar and hydrophilic compound separations

• Pharmaceutical and QC workflows

• Long‑chain fatty acids

• Mixed polarity sample analysis

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances retention of polar compounds

• Maintains classic C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable run‑to‑run reproducibility

Important Usage Notes

• Designed for 2.1 mm LC‑MS and low‑flow analytical systems

• Ideal for fast screening and high‑throughput workflows

• Suitable for high aqueous mobile phases (including 100% water)

• Ensure system capability for stable low‑flow operation (~0.2–0.3 mL/min)