Catalog Number - 84318-25P

The 84318‑25P Cogent RP C18 PE™ HPLC Column is engineered for maximum‑resolution analytical separations, combining 3 µm high‑efficiency particles with a polar embedded amide group within a C18 ligand to deliver superior retention of polar analytes while maintaining traditional hydrophobic selectivity.

Manufactured using high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol activity

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous mobile phases, including 100% water

The embedded amide functionality prevents phase collapse typical of conventional C18 columns, allowing reliable retention of polar compounds while maintaining excellent reversed‑phase characteristics.

250 mm Length – Maximum Separation & Highest Purity

• Highest resolving power in the analytical C18 PE column range

• Maximizes peak capacity for highly complex mixtures

• Ideal for closely related compounds and challenging separations

• Provides extended retention for hydrophobic analytes

• Best suited for final method development, validation, and highest‑purity results

This configuration is selected when no shorter column (50 mm or 100 mm) can achieve the required resolution.

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • quality control (QC)

  • regulated laboratory workflows

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Suitable for standard analytical systems without special flow requirements

3 µm Particle – High Efficiency Performance

• Higher efficiency than 5 µm columns of the same phase

• Produces sharper peaks and improved resolution

• Ideal for precise analytical separations and method optimization

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Operates in:

  • Reversed Phase (RP)

  • HILIC‑like behavior under specific conditions

Ideal for Challenging Separations

• Suitable for:

  • polar analytes with poor retention on conventional C18

  • mixed polarity sample sets

• Enables robust, flexible method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • consistent polar analyte retention

Typical Applications

• Analytical method development

• Pharmaceutical QC and validation

• Polar and hydrophilic compound separations

• Complex mixture analysis

• Long‑chain fatty acids

• Final method validation workflows

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances retention of polar analytes

• Maintains traditional C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable run‑to‑run results

Important Usage Notes

• Designed for maximum‑resolution analytical workflows

• Suitable for high aqueous mobile phases (including 100% water)

• Compatible with standard 4.6 mm ID HPLC systems

• Optimize conditions depending on RP or mixed‑mode operation