Catalog Number - 84318-15P-2

The 84318‑15P‑2 Cogent RP C18 PE™ HPLC Column is engineered for maximum‑resolution separations in low‑flow LC‑MS applications, utilizing 3 µm particles and a polar embedded amide group within a C18 ligand to deliver enhanced polar retention and high‑efficiency separations.

Manufactured using high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak symmetry for polar and basic analytes

• Stable retention under high aqueous conditions, including 100% water mobile phases

The embedded amide functionality allows stronger interaction with polar compounds, preventing phase collapse seen in traditional C18 columns, while retaining hydrophobic selectivity for non‑polar compounds.

150 mm Length – Maximum Resolution for LC‑MS Applications

• Highest resolving power in the 2.1 mm analytical PE column range

• Maximizes peak capacity for complex mixtures

• Ideal for separating closely related polar and hydrophobic compounds

• Preferred for final method optimization and high‑resolution LC‑MS workflows

• Longer run times compared to shorter columns

This configuration is selected when maximum separation performance is required beyond 50 mm and 100 mm formats.

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Designed for low‑flow HPLC and LC‑MS systems

• Enhances mass spectrometry sensitivity due to reduced solvent load

• Ideal for:

  • trace‑level detection

  • high‑sensitivity analytical methods

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires precise, stable low‑flow pumping systems and low‑volume detection hardware

3 µm Particle – High Efficiency Performance

• Higher efficiency than 5 µm columns of the same phase

• Produces sharper peaks and superior resolution

• Ideal for advanced analytical and LC‑MS workflows

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar analytes without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Can operate in:

  • Reversed Phase (RP)

  • HILIC‑like behavior under specific conditions

Ideal for Complex Samples

• Suitable for:

  • polar compounds with poor retention on conventional C18

  • mixed polarity samples

• Enables advanced method development flexibility

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • robust retention of polar analytes

Typical Applications

• LC‑MS analysis

• Polar and hydrophilic compound separations

• Pharmaceutical method development

• Metabolite and biomolecule analysis

• Peptides and small molecule separations

• Long‑chain fatty acids

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Improves retention of polar analytes

• Maintains traditional hydrophobic interaction capability

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable run‑to‑run reproducibility

Important Usage Notes

• Designed for 2.1 mm ID LC‑MS and low‑flow analytical systems

• Requires precise low‑flow pump control and small‑volume flow cells

• Ideal for maximum‑resolution LC‑MS workflows

• Suitable for high aqueous mobile phases (including 100% water)

• Optimize conditions depending on RP or mixed‑mode operation