Catalog Number - 84318-15P

The 84318‑15P Cogent RP C18 PE™ HPLC Column is engineered for maximum‑resolution analytical separations, combining 3 µm particles with a polar embedded amide group within a C18 ligand to deliver enhanced retention of polar analytes without sacrificing hydrophobic selectivity.

Manufactured on high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol activity

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous conditions, including 100% water mobile phases

The embedded amide functionality prevents phase collapse common with conventional C18 phases, while maintaining strong reversed‑phase performance for hydrophobic compounds.

150 mm Length – Maximum Resolution for Analytical Methods

• Highest resolving power in the analytical PE column range

• Maximizes peak capacity for complex mixtures

• Ideal for closely related compounds and difficult separations

• Preferred for final method development and high‑precision analysis

• Longer run times compared to shorter column formats

This configuration is selected when maximum separation performance is required beyond 50 mm and 100 mm columns.

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • quality control (QC)

  • routine and regulated workflows

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Suitable for standard analytical HPLC systems with no special flow requirements

3 µm Particle – High Efficiency Performance

• Higher efficiency than 5 µm columns of the same phase

• Produces sharper peaks and improved resolution

• Ideal for analytical precision and method optimization

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Operates in:

  • Reversed Phase (RP)

  • HILIC‑like behavior under appropriate conditions

Ideal for Challenging Separations

• Suitable for:

  • polar analytes poorly retained on traditional C18

  • mixed polarity sample matrices

• Enables flexible and robust method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • robust polar analyte retention

Typical Applications

• Analytical method development

• Pharmaceutical QC and testing

• Polar and hydrophilic compound separations

• Complex mixture analysis

• Long‑chain fatty acids

• Final method validation workflows

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Retains classic hydrophobic selectivity of C18

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable run‑to‑run reproducibility

Important Usage Notes

• Ideal for maximum‑resolution analytical workflows

• Suitable for high aqueous mobile phases (including 100% water)

• Compatible with standard 4.6 mm analytical systems

• Optimize based on RP or mixed‑mode separation behavior