Catalog Number - 84318-10P-2

The 84318‑10P‑2 Cogent RP C18 PE™ HPLC Column is engineered for improved retention of polar analytes while maintaining reversed‑phase selectivity, utilizing 3 µm particles and a polar embedded amide functional group within a C18 ligand.

Manufactured on high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous conditions, including 100% water mobile phases

The embedded amide group increases interaction with polar compounds while maintaining hydrophobic retention, enabling separation of compounds that are often poorly retained on standard C18 phases.

100 mm Length – Increased Resolution for LC‑MS

• Higher resolving power than 50 mm columns

• Improved separation of closely related compounds

• Increased peak capacity for analytical workflows

• Ideal for LC‑MS applications requiring better resolution and peak separation

This configuration is selected when greater separation performance is needed beyond initial fast screening.

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Designed for low‑flow HPLC and LC‑MS systems

• Enhances detection sensitivity in mass spectrometry

• Ideal for:

  • trace‑level analysis

  • high‑sensitivity workflows

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires HPLC/UHPLC systems capable of stable low‑flow operation

3 µm Particle – High Efficiency Performance

• Higher efficiency than 5 µm columns of the same phase

• Produces sharper peaks and improved resolution

• Ideal for analytical method development and LC‑MS workflows

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable under 100% aqueous mobile phases

• Improves retention compared to standard C18 phases

Dual‑Mode Flexibility

• Functions in:

  • Reversed Phase (RP)

  • HILIC‑like behavior under appropriate conditions

Ideal for Challenging Separations

• Suitable for:

  • polar analytes with poor retention on traditional C18

  • mixed polarity samples

• Enables flexible method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods using:

  • high aqueous mobile phase compositions

  • polar compound retention requirements

Typical Applications

• LC‑MS analysis

• Polar and hydrophilic compound separations

• Pharmaceutical method development

• Metabolite and small molecule analysis

• Peptides and biomolecule workflows

• Long‑chain fatty acids

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Maintains classic C18 hydrophobic selectivity

Fully End‑Capped Surface

• Reduces secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable run‑to‑run performance

Important Usage Notes

• Designed for 2.1 mm LC‑MS and low‑flow analytical systems

• Ideal for methods using high aqueous mobile phases (up to 100% water)

• Suitable for both RP and mixed‑mode separations

• Ensure system capability for stable low‑flow operation