Catalog Number - 84318-05P

The 84318‑05P Cogent RP C18 PE™ HPLC Column is designed for enhanced retention of polar analytes while maintaining traditional reversed‑phase performance, utilizing 3 µm particles with a polar embedded amide group within a C18 ligand.

Manufactured on high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous mobile phases, including 100% water

The embedded amine/amide functional group differentiates this phase from standard C18 by allowing additional interaction with polar compounds, increasing retention where conventional phases often fail.

50 mm Length – Fast Scouting & High‑Throughput Analysis

• Designed for rapid separations and short run times

• Ideal for method development and screening workflows

• Reduces solvent consumption

• Commonly used for preparative method scouting

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • QC and routine analysis

• Provides robust and reproducible analytical performance

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Suitable for standard analytical HPLC systems without special flow requirements

3 µm Particle – High Efficiency & Sharper Peaks

• Higher efficiency than 5 µm columns of the same phase

• Produces sharper peaks and better resolution

• Ideal for analytical precision and method optimization

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar analytes without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 phases

Dual‑Mode Flexibility

• Can operate in:

  • Reversed Phase (RP)

  • HILIC‑like mechanisms under appropriate conditions

Ideal for Challenging Separations

• Suitable for:

  • polar compounds with poor retention on standard C18

  • mixed polarity samples

• Excellent for method development versatility

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • robust polar analyte retention

Typical Applications

• Analytical method development

• Polar and hydrophilic compound separations

• Pharmaceutical QC and testing

• Preparative method scouting

• Long‑chain fatty acids

• Mixed polarity compound analysis

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances retention of polar compounds

• Maintains traditional hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable run‑to‑run reproducibility

Important Usage Notes

• Ideal for method development and scouting applications

• Suitable for 100% aqueous mobile phases

• Compatible with standard analytical HPLC systems (4.6 mm ID)

• Optimize method conditions depending on RP or mixed‑mode operation