Catalog Number - 84018-25P

The 84018‑25P Cogent RP C18 PE™ HPLC Column is engineered for maximum‑resolution analytical separations and full method scalability, combining 10 µm particles with a polar embedded amide group within a C18 ligand to deliver robust performance, enhanced polar retention, and reliable method transfer to preparative workflows.

Manufactured using high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak symmetry for polar and basic analytes

• Stable performance under high aqueous mobile phases, including 100% water

The embedded amide functionality enhances retention of polar compounds while preventing phase collapse associated with conventional C18 phases, maintaining consistent reversed‑phase behavior for hydrophobic analytes.

250 mm Length – Maximum Separation & Highest Purity

• Highest resolving power in the 10 µm PE analytical range

• Maximizes peak capacity for highly complex mixtures

• Ideal for closely related compounds and difficult separations

• Provides extended retention and improved separation of challenging analytes

• Best suited for final method development, validation, and highest‑purity results prior to scale‑up

This configuration is selected when shorter columns (50 mm or 100 mm) cannot achieve the required resolution or separation quality.

4.6 mm ID – Analytical to Preparative Bridge Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development and optimization

  • QC and analytical validation

  • transfer to preparative or process‑scale chromatography

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Serves as a direct bridge between analytical development and preparative scale‑up

10 µm Particle – Preparative Scouting & High‑Flow Capability

• Designed for high‑flow compatibility and durability

• Provides lower flow resistance compared to smaller particle sizes

• Supports seamless method transfer to larger ID preparative and process columns

✅ Ideal when scalability, robustness, and throughput are prioritized over analytical efficiency

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable under 100% aqueous mobile phase conditions

• Provides improved retention vs standard C18 columns

Dual‑Mode Flexibility

• Operates in:

  • Reversed Phase (RP)

  • HILIC‑like modes under appropriate conditions

Ideal for Method Development & Scale‑Up

• Suitable for:

  • polar analytes poorly retained on conventional C18

  • full workflow from analytical to preparative scale

• Enables robust and transferable method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • consistent retention of polar compounds

Typical Applications

• Analytical method development

• Final method validation and optimization

• Preparative method scouting and scale‑up

• Polar and hydrophilic compound separations

• Pharmaceutical process development

• Long‑chain fatty acids

• Complex and mixed polarity samples

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Maintains classic C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable method transfer across systems

Important Usage Notes

• Designed for maximum‑resolution analytical workflows and scale‑up preparation

• Suitable for high aqueous mobile phases (including 100% water)

• Compatible with standard 4.6 mm analytical HPLC systems

• Ideal for resolution‑critical methods prior to preparative or process implementation