Catalog Number - 84018-10P

The 84018‑10P Cogent RP C18 PE™ HPLC Column is engineered for robust analytical method development and preparative method scaling, utilizing 10 µm particles combined with a polar embedded amide group within a C18 ligand to provide stable polar retention and highly durable chromatographic performance.

Manufactured using high‑purity silica with fully double end‑capped bonding, this stationary phase delivers:

• Reduced silanol interactions

• Improved peak symmetry for polar and basic analytes

• Stable operation with high aqueous mobile phases, including 100% water

The embedded amide functionality enhances retention of polar compounds while preventing phase collapse typical of conventional C18 phases, maintaining reliable reversed‑phase performance for hydrophobic analytes.

100 mm Length – Method Development & Increased Resolution

• Higher resolving power than 50 mm scouting columns

• Improves separation of closely related compounds

• Increases peak capacity for method refinement

• Ideal for transitioning from screening to scalable purification methods

This configuration is selected when additional resolution is required before scaling to preparative columns.

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • routine analysis

  • preparative method transfer

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Suitable for standard analytical systems without special flow requirements

10 µm Particle – Preparative Scouting & High‑Flow Capability

• Designed for high flow rates and method scalability

• Provides excellent durability in repeated method development cycles

• Supports transfer from analytical to preparative workflows

✅ Ideal when method scalability and robustness are prioritized over analytical efficiency

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable under 100% aqueous mobile phase conditions

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Operates in:

  • Reversed Phase (RP)

  • HILIC‑like modes under appropriate conditions

Ideal for Method Development & Scale‑Up

• Suitable for:

  • polar analytes poorly retained on conventional C18

  • scalable method development workflows

• Enables consistent performance from analytical to preparative scale

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • reliable retention of polar compounds

Typical Applications

• Analytical method development

• Preparative method scouting and scale‑up

• Polar and hydrophilic compound separations

• Pharmaceutical process development

• Long‑chain fatty acids

• Mixed polarity compound analysis

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Maintains standard C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable method transfer

Important Usage Notes

• Designed for method development and preparative scouting workflows

• Suitable for high aqueous mobile phases (including 100% water)

• Compatible with standard 4.6 mm HPLC systems

• Ideal when transition to preparative or process‑scale chromatography is planned