HPLC Column, NP Silica, Wide Pore, 5um, 4.6mm ID x 150mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Silica™ Wide Pore HPLC Columns are designed for normal-phase and HILIC separations of larger polar and hydrophilic compounds. Manufactured from ultra-high purity, low-metal-content 300 Å silica, this stationary phase is completely unmodified, providing the selectivity associated with USP L3 classified columns. The wide pore structure improves accessibility for larger analytes, making these columns an excellent choice for proteins, peptides, biomolecules, and other high molecular weight compounds.
82561-15P-3M is packed with 5 µm NP Silica™ Wide Pore particles in a 4.6 mm ID x 150 mm format. The combination of a 300 Å pore size and unmodified silica surface provides traditional silica selectivity while enhancing mass transfer and accessibility for larger molecules. The 150 mm column length delivers increased resolving power and peak capacity, making it suitable for demanding analytical methods and complex separations.
The 4.6 mm ID x 150 mm format is well suited for HILIC applications, normal-phase chromatography, pharmaceutical analysis, quality control testing, and method development involving proteins, peptides, and other larger hydrophilic compounds. Typical flow rates are approximately 0.8-1.5 mL/min depending on mobile phase composition and application requirements.
Key Benefits
NP Silica™ Wide Pore stationary phase
USP L3 equivalent chemistry
Ultra-high purity, low-metal-content silica
Unmodified silica surface
300 Å wide pore structure
Suitable for HILIC applications
Suitable for normal-phase chromatography
Enhanced accessibility for larger molecules
Lower backpressure with 5 µm particles
Broad application versatility
Typical Applications
HILIC chromatography
Normal-phase HPLC
Protein analysis
Peptide analysis
Biomolecule separations
Polar compound analysis
Hydrophilic compound analysis
Pharmaceutical analysis
Method development
Quality control testing
Specifications
Product Type: HPLC Column
Stationary Phase: NP Silica™ Wide Pore
Chromatography Mode: HILIC / Normal Phase
USP Classification: L3
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 4.6 mm ID x 150 mm
Recommended Flow Rate: 0.8-1.5 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Suitable for HILIC applications
Suitable for normal-phase chromatography
Unmodified silica stationary phase
USP L3 equivalent chemistry
Wide pore design improves accessibility for proteins, peptides, and larger molecules
Excellent for biomolecule and high molecular weight compound analysis
Low-metal-content silica support
150 mm length provides increased resolution and peak capacity
Useful for method development and complex separations
Compatible with most standard HPLC systems