Catalog Number - 82560-05P-3M

Cogent NP Diol™ Wide Pore HPLC Columns are designed for normal-phase and HILIC separations of polar and hydrophilic compounds, including larger biomolecules that require enhanced pore accessibility. Manufactured using ultra-high purity 300 Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention, excellent stability, and broad solvent compatibility. The non-endcapped surface chemistry offers selectivity consistent with USP L20 classifications, making it an excellent choice for both established methods and new method development involving larger molecular weight analytes.

82560-05P-3M is packed with 5 µm NP Diol™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 50 mm format. The large pore structure improves accessibility for proteins, peptides, and other higher molecular weight compounds while maintaining excellent retention of hydrophilic and polar analytes. NP Diol™ Wide Pore columns are often selected as alternatives to unmodified silica columns because they provide improved robustness and compatibility with a wider range of solvents while maintaining similar retention characteristics for many applications.

The 4.6 mm ID x 50 mm format is ideal for rapid analytical methods, HILIC separations, normal-phase chromatography, method development, and routine laboratory analyses. Typical flow rates are approximately 0.8-1.5 mL/min depending on mobile phase composition and application requirements.

Key Benefits

• NP Diol™ Wide Pore stationary phase

• USP L20 equivalent chemistry

• Ultra-high purity 300 Å silica support

• Terminal diol functional group

• 300 Å wide pore structure

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Ideal for larger molecules, proteins, and peptides

• Alternative to unmodified silica columns

• Broad solvent compatibility

Typical Applications

• HILIC chromatography

• Normal-phase HPLC

• Protein analysis

• Peptide analysis

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• Carbohydrate analysis

• Biomolecule separations

• Method development

Specifications

• Product Type: HPLC Column

• Stationary Phase: NP Diol™ Wide Pore

• Chromatography Mode: HILIC / Normal Phase

• Functional Group: Diol

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 4.6 mm ID x 50 mm

• Recommended Flow Rate: 0.8-1.5 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and normal-phase chromatography

• Often used as an alternative to USP L20 columns

• Wide pore design improves accessibility for proteins, peptides, and larger molecules

• Provides retention of hydrophilic and polar compounds

• More robust than many unmodified silica columns

• Broad solvent compatibility for method flexibility

• Short 50 mm length minimizes run times and solvent consumption

• Ideal for rapid analytical methods and method development

• Useful for biomolecules, carbohydrates, metabolites, and other polar analytes

• 4.6 mm ID format is compatible with most standard HPLC systems