Catalog Number - 82560-05P-2-3M

Cogent NP Diol™ Wide Pore HPLC Columns are designed for normal-phase and HILIC separations of polar and hydrophilic compounds, including larger biomolecules that benefit from enhanced pore accessibility. Manufactured using ultra-high purity 300 Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention, excellent stability, and broad solvent compatibility. The non-endcapped surface chemistry offers selectivity consistent with USP L20 classifications, making it an excellent choice for both established methods and new method development involving larger molecular weight analytes.

82560-05P-2-3M is packed with 5 µm NP Diol™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 50 mm format. The large pore structure improves accessibility for proteins, peptides, and other higher molecular weight compounds while maintaining excellent retention of hydrophilic and polar analytes. The narrow internal diameter and short column length are ideal for LC-MS compatibility, low solvent consumption, and rapid analysis. NP Diol™ Wide Pore columns are often selected as alternatives to unmodified silica columns because they provide improved robustness and compatibility with a wider range of solvents while maintaining similar retention characteristics for many applications.

The 2.1 mm ID x 50 mm format is commonly used for LC-MS methods, biomolecule analysis, metabolite studies, pharmaceutical applications, and method development involving polar compounds. Typical flow rates are approximately 0.2-0.3 mL/min. Standard HPLC systems used with 2.1 mm ID columns must be capable of delivering stable and reproducible low flow rates for optimum chromatographic performance.

Key Benefits

• NP Diol™ Wide Pore stationary phase

• USP L20 equivalent chemistry

• Ultra-high purity 300 Å silica support

• Terminal diol functional group

• 300 Å wide pore structure

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Ideal for larger molecules, proteins, and peptides

• Alternative to unmodified silica columns

• Broad solvent compatibility

Typical Applications

• HILIC chromatography

• Normal-phase HPLC

• Protein analysis

• Peptide analysis

• Polar compound analysis

• Hydrophilic compound analysis

• LC-MS methods

• Pharmaceutical analysis

• Metabolite analysis

• Method development

Specifications

• Product Type: HPLC Column

• Stationary Phase: NP Diol™ Wide Pore

• Chromatography Mode: HILIC / Normal Phase

• Functional Group: Diol

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Recommended Flow Rate: 0.2-0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and normal-phase chromatography

• Often used as an alternative to USP L20 columns

• Wide pore design improves accessibility for proteins, peptides, and larger molecules

• Provides retention of hydrophilic and polar compounds

• More robust than many unmodified silica columns

• Broad solvent compatibility for method flexibility

• Short 50 mm length minimizes run times and solvent consumption

• Excellent choice for LC-MS applications

• Useful for biomolecules, carbohydrates, metabolites, and other polar analytes

• Standard HPLC systems must be capable of reliable low-flow operation at approximately 0.2-0.3 mL/min