Catalog Number - 82361-25P-3M

Cogent NP Silica™ Wide Pore HPLC Columns are designed for the separation of large hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured from ultra-high purity, low metal content 300Å silica that is not chemically modified, this stationary phase provides the native silica selectivity required for challenging separations involving larger molecules. NP Silica™ Wide Pore is widely used as an alternative for USP L3 methods involving biomolecules and other high molecular weight compounds, as well as for new analytical method development.

82361-25P-3M is packed with 3 µm NP Silica™ Wide Pore particles in a 4.6 mm ID x 250 mm analytical format. The extended 250 mm column length provides maximum retention, chromatographic resolution, and peak capacity for demanding analytical separations involving large molecules and complex sample matrices. The 3 µm particle size delivers higher chromatographic efficiency and sharper peak shapes than comparable 5 µm columns, although increased operating backpressure should be expected. This format is ideal when the highest level of separation performance is required.

NP Silica™ Wide Pore is commonly used for large hydrophilic and polar compounds in both HILIC and Normal Phase chromatography. Because the stationary phase is completely unmodified, it provides the classic selectivity associated with bare silica while the 300Å pore structure improves accessibility for larger analytes. This format is particularly useful for proteins, peptides, biomolecules, and other compounds that may experience restricted pore access with conventional pore-size silica materials.

Key Benefits

• NP Silica™ Wide Pore stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 300Å wide pore design

• 3 µm particle size

• Higher efficiency than 5 µm particles

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

Typical Applications

• USP L3 methods

• HILIC chromatography

• Normal Phase HPLC

• Large molecule analysis

• Protein analysis

• Peptide analysis

• Biomolecule separations

• Polar compound analysis

• Method development

• Complex analytical separations

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Silica™ Wide Pore

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 3 µm

• Pore Size: 300Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent chemistry

• Unmodified silica stationary phase

• Designed for large hydrophilic and polar compounds

• Low metal content, high-purity silica

• Wide pore 300Å silica provides improved accessibility for larger molecules

• Provides higher efficiency than comparable 5 µm columns

• Generates higher operating backpressure than 5 µm particles

• Compatible with standard analytical HPLC systems capable of supporting 3 µm particle columns

• Frequently used for large molecule and established silica-based methods

• Broad solvent compatibility

• 250 mm length provides maximum retention, resolution, and peak capacity

• Ideal for complex separations and challenging analytical methods

  ---