Catalog Number - 82361-25P

Cogent NP Silica™ HPLC Columns are designed for the separation of hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured from ultra-high purity, low metal content 100Å silica that is not chemically modified, this stationary phase provides the native silica selectivity required for many traditional Normal Phase and HILIC applications. NP Silica™ is widely used as an alternative for USP L3 methods and for new analytical methods requiring the unique retention characteristics of unbonded silica.

82361-25P is packed with 3 µm NP Silica™ particles in a 4.6 mm ID x 250 mm analytical format. The extended 250 mm column length provides maximum retention, chromatographic resolution, and peak capacity for demanding analytical separations. The 3 µm particle size delivers higher chromatographic efficiency and sharper peak shapes than comparable 5 µm columns, although increased operating backpressure should be expected. This format is ideal when the highest separation performance is required for complex mixtures and closely related compounds.

NP Silica™ is commonly used for hydrophilic and polar compounds in both HILIC and Normal Phase chromatography. Because the stationary phase is completely unmodified, it provides the classic selectivity associated with bare silica and is often selected for established USP methods, method transfer projects, and applications requiring strong polar interactions. The 250 mm length maximizes resolving power, making this column especially suitable for complex analytical methods and challenging separations.

Key Benefits

• NP Silica™ stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 3 µm particle size

• Higher efficiency than 5 µm particles

• Sharper peak shapes

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

Typical Applications

• USP L3 methods

• HILIC chromatography

• Normal Phase HPLC

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• Food and beverage testing

• Environmental analysis

• Method development

• Complex analytical separations

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Silica™

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 3 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent chemistry

• Unmodified silica stationary phase

• Designed for hydrophilic and polar compounds

• Low metal content, high-purity silica

• Provides higher efficiency than comparable 5 µm columns

• Generates higher operating backpressure than 5 µm particles

• Compatible with standard analytical HPLC systems capable of supporting 3 µm particle columns

• Frequently used for established silica-based methods

• Broad solvent compatibility

• 250 mm length provides maximum retention, resolution, and peak capacity

• Ideal for complex separations and challenging analytical methods

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